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Protonen-Transfer und Protein-Zustände Dynamics in Lichtempfindliche Proteine, die durch zeitaufgelöste Step-Scan Fourier-Transformations-Infrarot-Spektroskopie
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Protonen-Transfer und Protein-Zustände Dynamics in Lichtempfindliche Proteine, die durch zeitaufgelöste Step-Scan Fourier-Transformations-Infrarot-Spektroskopie
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June 27, 2014
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Key steps of protein function, in particular backbone conformational changes and proton transfer reactions, often take place in the microsecond to millisecond time scale. These dynamical processes can be studied by time-resolved step-scan Fourier-transform infrared spectroscopy, in particular for proteins whose function is triggered by light.
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Lórenz-Fonfría, V. A., Heberle, J. Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy. J. Vis. Exp. (88), e51622, doi:10.3791/51622 (2014).
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