Optogenetics has become a powerful tool for use in behavioral neuroscience experiments. This protocol offers a step-by-step guide to the design and set-up of laser systems, and provides a full protocol for carrying out multiple and simultaneous in vivo optogenetic stimulations compatible with most rodent behavioral testing paradigms.
Sidor, M. M., Davidson, T. J., Tye, K. M., Warden, M. R., Diesseroth, K., McClung, C. A. In vivo Optogenetic Stimulation of the Rodent Central Nervous System. J. Vis. Exp. (95), e51483, doi:10.3791/51483 (2015).