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Tomat / GFP-FLP / FRT Metode til Live Imaging af Mosaic Voksen<em> Drosophila</em> fotoreceptorceller
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Biologie
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Journal JoVE Biologie
The Tomato/GFP-FLP/FRT Method for Live Imaging of Mosaic Adult Drosophila Photoreceptor Cells

Tomat / GFP-FLP / FRT Metode til Live Imaging af Mosaic Voksen<em> Drosophila</em> fotoreceptorceller

DOI:
10.3791/50610-v

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09:33 min

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September 20, 2013

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, , , ,
1Laboratory of Molecular Biology of the Cell,Ecole Normale Supérieure de Lyon, 2INSERM U744, Institut Pasteur de Lille,Université Lille-Nord de France, 3Howard Hughes Medical Institute, Laboratory of Apoptosis and Cancer,The Rockefeller University

Chapitres

  • 00:05Titre
  • 02:14Preparing Drosophila for Photoreceptor Visualization
  • 04:29Visualizing the Photoreceptors
  • 06:01Time-course Visualization of Photoreceptor Cells
  • 07:14Results from a High-throughput Screen
  • 09:13Conclusion

Summary

Traduction automatique

Traduction automatique

Tomat / GFP-FLP / FRT metode indebærer at visualisere mosaik fotoreceptorceller i levende Drosophila. Det kan bruges til at følge de enkelte fotoreceptor celleskæbner i nethinden dage eller uger. Denne metode er ideel til undersøgelser af retinal degeneration og neurodegenerative sygdomme eller fotoreceptorer celle udvikling.

Tags

DrosophilaPhotoreceptor CellsEyeClonal AnalysisLive ImagingTomato/GFP-FLP/FRTCornea NeutralizationMosaicNeuronal DegenerationPlanar Cell Polarity

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