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焦磷酸测序的微生物鉴定和表征

Journal JoVE
Biologie

This content is Free Access.

Journal JoVE Biologie

Pyrosequencing for Microbial Identification and Characterization

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焦磷酸测序的微生物鉴定和表征

46,280 Views

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12:37 min

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August 22, 2013

DOI:

10.3791/50405-v

DOI:

10.3791/50405-v

•

12:37 min

August 22, 2013

•

19 Views

Patrick J. Cummings¹, Ray Ahmed², Jeffrey A. Durocher², Adam Jessen², Tamar Vardi², Kristina M. Obom¹

¹Center for Biotechnology Education, Krieger School of Arts and Sciences,Johns Hopkins University, ²Qiagen Sciences, Inc.

Summary

焦磷酸测序法是一种通用的技术，可用于确定细菌种类，区分细菌的菌株，并检测抗微生物药物抗性的基因突变，有利于微生物的基因组测序。在这段视频中，微生物扩增子，扩增子的焦磷酸测序，DNA序列分析的程序将被证明。

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