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嵌入后突触蛋白的免疫标记海马切片培养
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Neurosciences
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Journal JoVE Neurosciences
Post-embedding Immunogold Labeling of Synaptic Proteins in Hippocampal Slice Cultures
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嵌入后突触蛋白的免疫标记海马切片培养

16,929 Views

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13:50 min

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April 03, 2013

DOI:

10.3791/50273-v

DOI:
10.3791/50273-v

•

13:50 min
April 03, 2013

•

5 Views
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1Department of Cell Biology, Neurobiology and Anatomy,Medical College of Wisconsin , 2Department of Microbiology and Molecular Genetics,Medical College of Wisconsin

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Immunogold LabelingPost-embeddingSynaptic ProteinsHippocampal Slice CulturesElectron MicroscopyUltrastructural LocalizationOsmium TetroxideUranyl AcetateTannic AcidAntigen DetectionStructural Preservation

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Zhong, L., Brown, J. C., Wells, C., Gerges, N. Z. Post-embedding Immunogold Labeling of Synaptic Proteins in Hippocampal Slice Cultures. J. Vis. Exp. (74), e50273, doi:10.3791/50273 (2013).

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