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Simultaneous Pre- and Post-synaptic Electrophysiological Recording from Xenopus Nerve-muscle Co-cultures
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Neurosciences
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Journal JoVE Neurosciences
Simultaneous Pre- and Post-synaptic Electrophysiological Recording from Xenopus Nerve-muscle Co-cultures
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Simultaneous Pre- and Post-synaptic Electrophysiological Recording from Xenopus Nerve-muscle Co-cultures

DOI:
10.3791/50253-v

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08:13 min

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March 11, 2013

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, , ,
1Department of Physiology,David Geffen School of Medicine at UCLA, 2Natural Science Division,Pepperdine University

Chapitres

  • 00:05Titre
  • 01:41Pre-experimental Preparation
  • 03:24Microdissection of Xenopus embryos
  • 04:51Preparation of Nerve-muscle Co-cultures
  • 05:52Patch Clamping Nerve-muscle Synapses
  • 07:02Results: Simultaneous Pre- and Post-synaptic Electrophysiological Recording from Xenopus Nerve-muscle Co-cultures
  • 07:36Conclusion

Summary

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This video demonstrates the procedures used to grow primary cultures of embryonic Xenopus nerve and muscle cells and the usefulness of this preparation for making simultaneous pre- and post-synaptic patch clamp recordings.

Tags

XenopusNerve-muscle Co-cultureElectrophysiologyPresynapticPostsynapticNeurotransmitter ReleaseIon ChannelsSynaptic InteractionsSynaptic DevelopmentSynaptic Plasticity

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