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Simultaneous Whole-cell Recordings from Photoreceptors and Second-order Neurons in an Amphibian Retinal Slice Preparation
Journal JoVE
Neurosciences
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Journal JoVE Neurosciences
Simultaneous Whole-cell Recordings from Photoreceptors and Second-order Neurons in an Amphibian Retinal Slice Preparation

Simultaneous Whole-cell Recordings from Photoreceptors and Second-order Neurons in an Amphibian Retinal Slice Preparation

DOI:
10.3791/50007-v

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11:39 min

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June 01, 2013

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,
1Department of Ophthalmology and Visual Sciences,University of Nebraska Medical Center , 2Department of Pharmacology and Experimental Neuroscience,University of Nebraska Medical Center

Chapitres

  • 00:05Titre
  • 01:57Retinal Slices Preparation
  • 06:13Paired Whole-cell Recordings
  • 09:21Results: Simultaneous Whole-cell Recordings from Photoreceptors and Second-order Neurons in an Amphibian Retinal Slice Preparation
  • 11:00Conclusion

Summary

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We describe the preparation of thin retinal slices from aquatic tiger salamanders (Ambystoma tigrinum) and explain how we use these slices to study synaptic processing in the retina by obtaining dual whole-cell voltage clamp recordings from photoreceptors and second-order horizontal and bipolar cells.

Tags

Retinal NeuroscienceRetinal CircuitryPhotoreceptorsRetinal NeuronsElectrophysiological RecordingVertical Retinal SliceDual Whole-cell Voltage ClampPhotoreceptor Membrane PotentialSynaptic RibbonNeurotransmitter Release

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