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Ex vivo Live Imaging of Single Cell Divisions in Mouse Neuroepithelium
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Neurosciences
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Journal JoVE Neurosciences
Ex vivo Live Imaging of Single Cell Divisions in Mouse Neuroepithelium

Ex vivo Live Imaging of Single Cell Divisions in Mouse Neuroepithelium

DOI:
10.3791/4439-v

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06:41 min

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April 30, 2013

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,
1Department of Human Genetics,Emory University School of Medicine, 2Department of Experimental Embryology,IGAB Polish Academy of Sciences

Chapitres

  • 00:05Titre
  • 01:13Embryo Generation
  • 02:09Whole Mouse Embryo Culture and Viral Infection
  • 03:27Neural Tube Slice Preparation and Live Imaging
  • 05:14Representative Live Imaging Results
  • 06:20Conclusion

Summary

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Traduction automatique

Here we develop the tools necessary for ex vivo live imaging to trace single cell divisions in the mouse E8.5 neuroepithelium

Tags

Ex Vivo Live ImagingSingle Cell DivisionsMouse NeuroepitheliumFluorescent MarkersCulturing SlicesEmbryonic Mouse NeuroepitheliumGenetic Cell Lineage TracingTamoxifen-inducible Cre LineCre Reporter LineDsRed ReporterCre-mediated RecombinationTamoxifen InductionIndividual Cell DivisionsTranscriptional ResponseSonic Hedgehog SignalingOlig2-eGFP Transgenic LineCilia MarkerSstr3-GFP ReporterNeuroepithelium ImagingTime-lapse Confocal ImagingPrimary Cilia FormationShh Response

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