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Rapid Genetic Analysis of Epithelial-Mesenchymal Signaling During Hair Regeneration
Journal JoVE
Biologie
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Journal JoVE Biologie
Rapid Genetic Analysis of Epithelial-Mesenchymal Signaling During Hair Regeneration

Rapid Genetic Analysis of Epithelial-Mesenchymal Signaling During Hair Regeneration

DOI:
10.3791/4344-v

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10:09 min

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February 28, 2013

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, , ,
1Program in Epithelial Biology,Stanford University School of Medicine

Chapitres

  • 00:05Titre
  • 01:31Isolating and Preparing Mouse Skins
  • 03:17Isolating Primary Dermal or Epidermal Cells from Skins
  • 05:07Grafting Primary Dermal and Epidermal Cells
  • 07:57Histological Analysis of Hair Follicle Growth
  • 09:32Conclusion

Summary

Traduction automatique

Traduction automatique

Tissue-specific analysis of a hair follicle regeneration model using lentivirus to mediate gain- or loss-of-function.

Tags

Genetic AnalysisEpithelial-mesenchymal SignalingHair RegenerationHair Follicle MorphogenesisOrgan DevelopmentLentivirusOverexpressionShRNA-mediated KnockdownGain-of-function AnalysisLoss-of-function AnalysisPrimary Mouse KeratinocytesDermal CellsDermal Papilla CellsHair FolliclesNude MiceGenetic Models

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