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Chapitres
- 00:05Titre
- 01:29Coupling Antibodies to MagPlex Microspheres, using the xMAP Antibody Coupling Kit
- 04:58Antibody Screening
- 07:20xMAP Functional Assay
- 09:07ELISA Assay
- 09:44Results: Comparison of an ELISA Assay for TNF-alpha Cytokine to the Converted Assay using the xMAP Platform
- 14:28Conclusion
En ELISA let kan omdannes til en Luminex xMAP assay og gennem fordelene ved multipleksing flere antistoffer kan screenes samtidigt at identificere en optimal antistof par, hvilket resulterer i forøget følsomhed og dynamikområde, og samtidig reducere assay omkostninger.
Tags
ELISALuminex XMAP AssayAntibody ScreeningMultiplexCapture AntibodyAnalytesBiological SamplesLife Science ResearchClinical DiagnosticsLimitations96-well MicroplateSample VolumeAntigenNon-specific BindingBackgroundEnzyme-mediated AmplificationSensitivityTechnologyHigher ThroughputFlexibilityReduced CostWorkflowLuminex XMAP TechnologyMicrosphere Array PlatformMonoplex AssayProtein ApplicationsNucleic Acid Applications
