Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Chapitres
- 00:05Titre
- 01:42Poly-D-Lysine: Preparation and Coating of the Plastic Cell Culture Dishes
- 03:03PDL and Laminin: Preparation and Coating of the Glass Two-chamber Slides
- 04:07Neuronal Dissection and Culture
- 09:40Results: Isolation and Culture of Rat Embryonic Neural Cells
- 10:27Conclusion
Tags
IsolationCultureRat Embryonic Neural CellsQuick ProtocolDissociationHippocampal NeuronsCortical NeuronsE15-17 Rat EmbryosMouse Primary NeuronsHuman Primary NeuronsNeural ProgenitorsSerum-free MediumNucleofectionImmunocytochemistryNucleic Acids PreparationElectrophysiologyLentiviral TransductionCalcium Phosphate TransfectionLipid-based Transfection
