This content is Free Access.
监视依赖黄素单加氧酶和氧化还原半反应，用停流分光光度法
14,824 Views
•
12:08 min
•
March 18, 2012
DOI:
Summary
Automatically generated
我们描述了一停流仪器的使用调查还原和氧化半反应<em>曲霉</em>一个铁载体（SIDA），黄素依赖型单加氧酶。然后，我们显示相应的在思达反应的物种的光谱，并计算其形成的速率常数。
Explore More Videos
Stopped-flow SpectrophotometryFlavin-dependent MonooxygenaseAspergillus Fumigatus Siderophore A (SidA)Hydroxylation Of OrnithineBiosynthesis Of Hydroxamate SiderophoresReductive Half-reactionOxidative Half-reactionFADNADPHC4a-hydroperoxyflavin IntermediateMeasurement Of RatesSpectral FormsStopped-flow InstrumentAnaerobic Glove BoxSingle ModeDouble Mixing Settings
Read Article
Cite this Article
Romero, E., Robinson, R., Sobrado, P. Monitoring the Reductive and Oxidative Half-Reactions of a Flavin-Dependent Monooxygenase using Stopped-Flow Spectrophotometry. J. Vis. Exp. (61), e3803, doi:10.3791/3803 (2012).
Copy