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鼠标感觉神经支配的皮肤和四肢骨骼纤维组织的制备及免疫染色
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Neurosciences
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Journal JoVE Neurosciences
Tissue Preparation and Immunostaining of Mouse Sensory Nerve Fibers Innervating Skin and Limb Bones
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鼠标感觉神经支配的皮肤和四肢骨骼纤维组织的制备及免疫染色

22,896 Views

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10:25 min

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January 26, 2012

DOI:

10.3791/3485-v

DOI:
10.3791/3485-v

•

10:25 min
January 26, 2012

•

5 Views
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1Department of Pharmacology,The University of Iowa, 2Department of Anesthesia,Roy J. and Lucille A. Carver College of Medicine, The University of Iowa

Summary

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末梢感觉神经纤维亚型的免疫细胞化学鉴定（和蛋白表达其中的检测）底层外围感觉的分子机制的理解是关键。在这里，我们描述外设/内脏组织样本，特定的免疫外周感觉神经纤维，如皮肤和四肢骨骼的制备方法。

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Shepherd, A. J., Mohapatra, D. P. Tissue Preparation and Immunostaining of Mouse Sensory Nerve Fibers Innervating Skin and Limb Bones. J. Vis. Exp. (59), e3485, doi:10.3791/3485 (2012).

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