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哺乳动物细胞培养,使用一种新的多层瓶中的比例增加
Journal JoVE
Biologie
This content is Free Access.
Journal JoVE Biologie
Scale-Up of Mammalian Cell Culture using a New Multilayered Flask
DOI:

08:39 min

December 05, 2011

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Chapitres

  • 00:05Titre
  • 01:00Design and Preparation of Falcon Multi-Flasks and Addition of Cell Suspensions
  • 02:19Mixing of Cells: Equilibration and Partitioning of Fluids
  • 03:23Transportation and Placement of the Multi-Flask
  • 04:12Media Exchange and Cell Harvesting
  • 05:40Recommended Working Volume in Falcon Multi-Flasks
  • 06:10Homogenous Cell-Population Generation
  • 08:25Conclusion

Summary

Traduction automatique

细胞发挥在研究的工具和越来越大的作用,并发现和开发新疗法。有了这个我们需要更有效率和更有效的方式,为种植和收获附件依赖细胞的细胞数量增加的需要。一个正确的功能的多层瓶中可以达到这个目的。

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