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Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3’UTR-reporter Constructs and a microRNA Mimic in Adherent Cells

Journal JoVE
Biologie

This content is Free Access.

Journal JoVE Biologie

Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3’UTR-reporter Constructs and a microRNA Mimic in Adherent Cells

Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3’UTR-reporter Constructs and a microRNA Mimic in Adherent Cells

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07:19 min

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September 28, 2011

DOI:

DOI:

•

07:19 min

September 28, 2011

•

2 Views

Shelley Force Aldred, Patrick Collins, Nathan Trinklein

¹SwitchGear Genomics

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LightSwitch Luciferase Assay System 3’UTR-reporter Constructs MicroRNAs (miRNAs)Gene Expression Regulators Computational Tools Expression Assays Proteomics Assays Chromatin-immunoprecipitation-based Techniques MiRNA-3’UTR Interactions Functional Evidence Cell-based System Genome-wide Collection Of Human 3’UTR Luciferase Reporters Synthetic MiRNA Target Reporter Constructs Endogenous 3’UTR Reporter Constructs Standardized Experimental Protocols Co-transfection MiRNA Mimic High-throughput Analysis

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