This content is Free Access.
Isolering og karakterisering af RNA-Varer Exosomes
Chapitres
- 00:05Titre
- 01:22Exosome Isolation
- 02:53Exosome Identification by Electron Microscopy
- 03:23Exosome Detection and Characterization by Flow Cytometry
- 04:49Exosome Detection and Characterization by Western Blot
- 06:08Isolation and Analysis of Exosomal RNA
- 07:13Results: Exosome Analyses
- 08:49Conclusion
Dette papir demonstrerer metoder til isolering, oprensning og detektion af exosomes, samt teknikker til analyse af deres molekylære indhold. Disse metoder er tilpasses til exosome isolation fra både cellekulturmedier og biologiske væsker, og kan udover analyse af molekylære indhold også være nyttige i funktionelle studier.
Tags
Exosome ResearchRNA-containing ExosomesVesiclesEndocytic OriginTransferrin ReceptorLate EndosomesMultivesicular Bodies (MVBs)Plasma Membrane FusionCell Release Of ExosomesBiological FluidsContent And Function Of ExosomesTolerance InductionTumor EradicationNatural Killer CellsT Regulatory CellsMRNA And MicroRNA In Exosomes
