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多光子成像在口腔鳞状细胞癌原位小鼠模型的肿瘤细胞的侵袭
Journal JoVE
Médecine
This content is Free Access.
Journal JoVE Médecine
Multi-photon Imaging of Tumor Cell Invasion in an Orthotopic Mouse Model of Oral Squamous Cell Carcinoma
DOI:

12:03 min

July 25, 2011

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Chapitres

  • 00:05Titre
  • 01:46Cell Lines, Vector Construction, and Lentiviral Production
  • 03:19Production of Head and Neck Cell Lines with Stable LifeAct-mCherry Expression
  • 04:30Orthotopic Tumor Xenograft Formation
  • 05:56Preparation of Mouse Tongues for Ex-vivo Imaging
  • 06:27Imaging of Tongue Tumors with Two-photon Microscopy and Image Analysis
  • 09:55Representative Tumor Images
  • 11:04Conclusion

Summary

Traduction automatique

产生的口腔癌和肿瘤浸润的定量监测的小鼠模型,通过多光子显微镜标记细胞内的舌头所涉及的技术的一个全面的概述。该系统可以作为一个有用的平台和反侵入性化合物的分子评估药物疗效。

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