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Generation of Composite Plants in Medicago truncatula used for Nodulation Assays
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Biologie
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Journal JoVE Biologie
Generation of Composite Plants in Medicago truncatula used for Nodulation Assays

Generation of Composite Plants in Medicago truncatula used for Nodulation Assays

DOI:
10.3791/2633-v

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13:37 min

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March 27, 2011

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, , ,
1Donald Danforth Plant Science Center

Chapitres

  • 00:02Titre
  • 00:05Introduction
  • 02:39Preparing Plant Materials
  • 06:09Generating Hairy Root Composite Plants
  • 09:39Symbiotic Rhizobia Infection
  • 11:10Transgenic Root Identification
  • 12:00Conclusion

Summary

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Traduction automatique

We demonstrate how hairy root composite plants can be used to study plant-rhizobium interactions and nodulation in the difficult-to-transform species Medicago truncatula.

Tags

Composite PlantsMedicago TruncatulaNodulation AssaysAgrobacterium RhizogenesHairy RootsTransgenic RootsGenetic EngineeringBroad Host RangeTransformationRecalcitrant Plant SpeciesGene FunctionsRoot-related Phenotypes

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