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1Cardiovascular Research Institute,University of California San Francisco, 2Department of Pediatrics,University of California San Francisco, 3Department of Biology,San Francisco State University, 4Department of Medicine,University of California San Francisco , 5Eli and Edythe Broad Center of Regeneration Medicine & Stem Cell Research,University of California San Francisco
Chapitres
- 00:05Titre
- 02:03Dissecting the Mouse Extensor Digitorum Longus Muscle
- 02:58Mounting the EDL in the Muscle Strip Myograph
- 03:51Twitch Tension Analysis
- 05:00Tetanic Tension, Force-Frequency, and Fatigue
- 06:18Additional Data Collection
- 06:46Results: Force-Frequency and Fatigue Analysis
- 07:53Conclusion
Per valutare l'<em> In vivo</em> Effetti degli interventi terapeutici per le malattie muscolari, i metodi sono necessari per quantificare costituzione della forza e affaticamento nei muscoli trattati. Noi dettaglio un approccio alla valutazione myo-meccaniche nel muscolo del mouse espiantati arti posteriori. Questa analisi fornisce un valido approccio per la quantificazione degli effetti delle modificazioni genetiche sulla funzione muscolare, così come il confronto di terapie in modelli murini di malattia muscolare.
Tags
Myo-mechanical AnalysisIsolated Skeletal MuscleTherapeutic InterventionsMuscle DiseaseQuantitative MethodsForce GenerationFatigabilityHindlimb MuscleExplanted MuscleMuscle Strip MyographMaximal Twitch TensionTetanic TensionContraction TimeHalf-relaxation TimeSquare Pulse StimulatorSpecific Twitch TensionSpecific Tetanic TensionMuscle Cross-sectional AreaTwitch-to-tetanic Tension RatioForce-frequency Relationship CurveLow Frequency Fatigue CurveComparison Between Therapeutic InterventionsMouse Models Of Muscle DiseaseGenetic ModificationMuscle Function
