Utilizing Multielectrode Arrays to Measure Synaptic Physiology in 3D Coculture Spheres
Transcription
Take a sterile multielectrode array or MEA, a device that records the electrical activity of target cells.
Add a polymer solution to the MEA and incubate.
The polymer coats the surface of the MEA, thereby increasing the water-attracting property of the surface.
Remove the excess polymer and wash with deionized water.
Add extracellular matrix, or ECM, and incubate to allow it to coat the MEA. Remove the excess ECM.
Next, add the 3D coculture spheres comprising neurons and astrocytes in a suitable media.
Incubate to allow the spheres to adhere to the surface of the MEA.
Place the MEA on a temperature-controlled head stage of an MEA system and record the spontaneous electrical activity across the electrodes.
The measured electrical activity corresponds to the communication at the neural junctions within the sphere.
