Journal
/
Biologie
/
Patch Clamp and Perfusion Techniques for Studying Ion Channels Expressed in Xenopus oocytes
Journal JoVE
Biologie
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Biologie
Patch Clamp and Perfusion Techniques for Studying Ion Channels Expressed in Xenopus oocytes

Patch Clamp and Perfusion Techniques for Studying Ion Channels Expressed in Xenopus oocytes

DOI:
10.3791/2269-v

•

10:19 min

•

January 10, 2011

•

, , ,
1Department of Energy, Environmental & Chemical Engineering,Washington University in St. Louis, 2Department of Biomedical Engineering,Washington University in St. Louis, 3Department of Biomedical Engineering and Cardiac Bioelectricity and Arrhythmia Center,Washington University in St. Louis

Chapitres

  • 00:05Titre
  • 00:45Introduction
  • 01:10Injecting mRNA into Oocytes
  • 01:51Preparing the Perfusion System
  • 04:26Preparing the Ag/AgCl Wire and the Data Acquisition Equipment
  • 05:33Stripping the Vitelline Membrane from the Oocyte
  • 06:14Preparing the Patch Pipettes
  • 07:06Obtaining an Inside-Out Patch
  • 08:08Patch Clamping
  • 08:41Recording of Wild-Type BK Channels on and Oocyte Membrane Patch
  • 09:57Conclusion

Summary

Traduction automatique

Traduction automatique

Ionic current of BK channels is recorded using patch clamp techniques. BK channels are expressed in Xenopus oocytes by injecting messenger RNA. The intracellular solution during patch clamp recordings is controlled by a perfusion system.

Tags

Patch ClampPerfusion TechniquesIon ChannelsXenopus OocytesBK ChannelsActivationStructure-function RelationshipNeurotransmitter ReceptorsSmooth Muscle ContractionHair Cell Frequency TuningNeurotransmitter ReleaseMembrane DepolarizationIntracellular Ca2+ And Mg2+Messenger RNA InjectionIncubationInside-out ConfigurationSingle Or Multiple ChannelsRecording CurrentsControlled Voltage

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

Methods for Patch Clamp Capacitance Recordings from the Calyx

Patch Clamp Recording of Ion Channels Expressed in Xenopus Oocytes

Pressure-polishing Pipettes for Improved Patch-clamp Recording

Microinjection of Xenopus Laevis Oocytes

Visualizing RNA Localization in Xenopus Oocytes

Mutagenesis and Functional Analysis of Ion Channels Heterologously Expressed in Mammalian Cells

Giant Liposome Preparation for Imaging and Patch-Clamp Electrophysiology

The Xenopus Oocyte Cut-open Vaseline Gap Voltage-clamp Technique With Fluorometry

One-channel Cell-attached Patch-clamp Recording

Reconstitution of a Transmembrane Protein, the Voltage-gated Ion Channel, KvAP, into Giant Unilamellar Vesicles for Microscopy and Patch Clamp Studies

Read Article