This protocol describes a method for micron-scale three-dimensional imaging of oxygen concentration in the immediate environment of live cells by electron spin resonance microscopy.
Halevy, R., Shtirberg, L., Shklyar, M., Blank, A. Electron Spin Resonance Micro-imaging of Live Species for Oxygen Mapping. J. Vis. Exp. (42), e2122, doi:10.3791/2122 (2010).