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Purification of Nuclei from Mouse Bone Marrow Hematopoietic Stem and Progenitor Cells (HSPCs)
Journal JoVE
Biologie
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Journal JoVE Biologie
Purification of Nuclei from Mouse Bone Marrow Hematopoietic Stem and Progenitor Cells (HSPCs)

Purification of Nuclei from Mouse Bone Marrow Hematopoietic Stem and Progenitor Cells (HSPCs)

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03:48 min

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December 22, 2023

DOI:

10.3791/200693-v

DOI:
10.3791/200693-v

•

03:48 min
December 22, 2023

•

3 Views
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1Cytometry and Biomarkers UTechS, C2RT,Institut Pasteur, Université Paris Cité, 2Microenvironment and Immunity Unit,Institut Pasteur, Université Paris Cité, INSERM U1224, 3Istituto Superiore di Sanità

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Nuclei PurificationMouse Bone MarrowHematopoietic Stem And Progenitor Cells (HSPCs)ACK Lysing BufferCell StrainerFACS BufferCell CountingCell StainingFlow CytometryCell SortingCollection MediumPBSBSA

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Purification of Nuclei from Mouse Bone Marrow Hematopoietic Stem and Progenitor Cells (HSPCs). J. Vis. Exp. (Pending Publication), e200693, doi:10.3791/200693 (2023).

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