电刺激在大鼠中建立急性庞廷梗死
Summary
此处介绍的是通过单个脉冲的电刺激在大鼠模型中建立急性庞汀梗死的协议。
Abstract
庞汀梗死是后循环中最常见的中风亚型，而缺乏模仿庞汀梗死的啮齿动物模型。提供此为成功建立急性庞汀梗死大鼠模型的协议。使用重约250克的老鼠，使用立体气器将带绝缘护套的探针注入到庞体内。病变是由单个脉冲的电刺激产生的。Longa 分数、伯德森分数和梁平衡测试用于评估神经缺陷。此外，粘合去除体感测试用于确定感官运动功能，肢体放置测试用于评估自感知。然后使用 MRI 扫描来评估体内的梗塞，TTC 染色用于确认体外梗死。在这里，一个成功的梗死被识别，位于栖息的龙的外端基础。最后，提出了一种建立急性庞汀梗死大鼠模型的新方法。
Introduction
自 20 世纪 80 年代以来，硅胶导的中脑动脉闭塞（ MCAO ）模型在基础中风研究中得到了广泛的应用。还使用了其他方法（即：MCA2的一个分支的苏化和光化学诱发的焦梗死）。这些模型被人们从中可看做为基于MCA的中风模型，对研究中风和潜在治疗的病理生理机制大有贡献。虽然这些实验模型3、4,有局限性，但这些方法已被许多实验室5、6,使用。基于 MCA 的笔画模型表示前循环中的笔画;然而，很少有报道调查了模型模仿中风的后循环7。
前循环中风的病因、机理、临床表现和预后有显著差异。因此，前循环笔触模型得出的结果不能应用于后循环笔画。例如，前循环的再灌注时间窗口已延长至6小时，根据成像结果9，研究部分延长至24小时。然而，根据之前的报告10 和我们自己的临床经验，后循环的时间窗口可能超过24小时。这个拉长的再灌注时间窗口必须在实验模型中进一步研究和确认。
关于后循环中风，庞汀梗塞是最常见的亚型，占所有缺血性中风病例的71例，12例。根据梗塞地形，庞汀梗塞分为隔离和非隔离的庞汀梗塞13。孤立的庞汀梗塞根据基本机制分为三种类型：大动脉疾病（LAD）、罗勒动脉分支病（BABD）和小动脉疾病（SAD）。对14例庞汀梗死的临床调查，对庞汀梗死的机制、表现和预后有一些了解。然而，模仿庞汀梗死的啮齿动物模型却较少受到研究。
在以前的研究中，涉及庞的扩散脑干分段损伤已被探索7。一组试图通过连接罗勒动脉（BA）15，创建一个庞汀梗死模型。另一组使用10-0尼龙单丝缝合有选择地将近极BA的四点选择性地（16）的四点。此模型模仿 LAD， 但大多数庞廷梗死源于 BABD 和 SAD。此外，BA的选择性结扎是一个复杂的手术，具有很高的死亡率。
此处提供了一个详细的协议，用于通过电刺激进行易于执行、易于复制和成功的大鼠模型急性庞汀梗死。
Protocol
Representative Results
Discussion
本研究为生成急性庞汀梗死大鼠模型提供了一种方案。该模型可用于研究蓬汀中风患者的预后和康复（包括中风后慢性疼痛）。
这种方法有几个优点。首先，它为今后的研究提供了急性庞汀梗死的老鼠模型。如上所述，庞汀梗塞是一种常见的中风亚型，受到的关注较少。中风研究的一个主要缺点是缺乏特定的庞汀梗死模型。其次，与BA15、16的结扎现有庞汀?…
Divulgations
The authors have nothing to disclose.
Acknowledgements
这项研究得到了中国自然科学基金（81471181和81870933）和江苏省自然科学基金（第81601011号）的财政支持（BK20160345）给朱J和广州市卫生局科学计划（20191A011083）给邱Z.
Materials
| 4-0 sucture | Shanghai Jinzhong | Surgical instruments | |
| Adhesive tape | Shanghai Jinzhong | Surgical instruments | |
| Animal anesthesia system | RWD | Wear mask when using the system | |
| Bone cement | Shanghai Jinzhong | Surgical instrument | |
| Cured clamp | Shanghai Jinzhong | Surgical instrument | |
| General tissue scissors | Shanghai Jinzhong | Surgical instrument | |
| IndoPhors | Guoyao of China | Sterilization | |
| Isoflurane | RWD | 217181101 | |
| Lesion Making Device | Shanghai Yuyan | Making a lesion | |
| MRI system | Bruker Biospin | Confirmation of infarction in vivo | |
| Needle holder | Shanghai Jinzhong | Surgical instrument | |
| Penicilin | Guoyao of China | Infection Prevention | |
| Probe | Anke | Need some modification | |
| Q-tips | Shanghai Jinzhong | Surgical instrument | |
| Shearing scissors | Shanghai Jinzhong | Surgical instrument | |
| Stereotaxic apparatus | RWD | ||
| Suture needle | Shanghai Jinzhong | Surgical instrument | |
| Tissue holding forcepts | Shanghai Jinzhong | Surgical instrument | |
| TTC | Sigma-Aldrich | BCBW5177 | For infarction confirmation in vitro |
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