Viral Tracing of Genetically Defined Neural Circuitry

1. Making Virus from cDNA: Recovery of VSV from cDNA using Vaccinia-T7 System¹⁰

1. One day prior to experiment, split BsrT7 cells into 60 mm dish containing DMEM + 10% FBS. Seed 2E6 cells per dish. BsrT7 cells are derived from BHK21, or baby hamster kidney, cells.
2. Warm PBS with 1 mM magnesium and 1 mM calcium to 37 °C.
3. Obtain a small aliquot vTF7-3, a vaccinia virus expressing T7 polymerase, and thaw to room temperature. vTF7-3 is an infectious vaccinia virus expressing the T7 polymerase¹¹, and should be used only in Biosafety level 2 containment. This expression system is used to generate maximal levels of the desired transcripts from plasmids transfected in step 1.9. Vortex virus for 30 sec at max speed. Sonicate in a tabletop sonicator for 2 min in water bath at room temperature. Vortex again for 30 sec to break up viral clumps.
4. Remove media from the BSRT7 cells.
5. Add 11.7 μl vTF7-3 to 600 μl PBS with magnesium and calcium and add mixture onto the cells.
6. Move plates into a 34 °C incubator with 5% CO₂. Rock plates gently once every 10 min.
7. After 45 min have elapsed, begin transfection. Add 20 μl lipofectamine 2,000 to 1 ml DMEM and mix. Wait 5 min.
8. Add 5.25 μg pN, 2.3 μg pP, 1.2 μg pl, 1 μg pCAG RABV-G, and 6 μg VSV genomic cDNA to 500 μl DMEM. pN, pP and pl are plasmids driving expression of VSV viral genes under the T7 promoter¹⁰, with the amounts determined empirically for optimum rescue of the virus.
9. Combine both tubes and mix. Wait 15 min at room temperature.
10. Remove PBS and wash the plates once with 1.5 ml DMEM.
11. Aspirate rinse media. Add transfection mix to plate. Move plates into 34 °C incubator.
12. Wait 5 hr, then remove media.
13. Change media to 4 ml DMEM + 2% FBS + 1x Penicillin-Streptomycin + 10 mM HEPES pH 7.4. The addition of Penicillin-Streptomycin, to prevent contamination, and HEPES, to maintain the pH of the media, are very important. The FBS content can be dropped to 2%, as the cells will be destroyed in a matter of days by the virus and thus do not need 10% FBS.
14. Place plates into 34 °C incubator for 48-72 hr.
15. Collect media at 3 and 6 days post-transfection, and immediately syringe filter through a 0.20 μm filter. This size filter removes the vaccinia virus, but not the VSV.
16. Since we want to supply RABV-G to the viral envelope, and the genome does not encode the RABV-G gene, it must be continually supplied in trans. To do this, make a separate plate of 293T TVA800 (TVA800) cells. These are cells that constitutively express the TVA receptor, permitting EnvA-mediated viral infection¹². At 80% confluency, change media to DMEM only, then transfect the cells with 5 μg of plasmid encoding G protein (i.e. pCAG RABV-G) per plate. We use the PEI transfection reagent, a polycationic polymer which is much cheaper than comparable lipid-based methods. However, lipofectamine can also be used effectively for this purpose. The optimum PEI : DNA ratio needs to be determined empirically. Do this in a separate experiment with a fluorescent reporter, i.e. pCAG GFP. We use 10 μl PEI: 4 μg DNA.
17. Apply the supernatant from step 16 onto the transfected plate of TVA800 cells.
18. Observe this plate the following day for evidence of fluorescence of the virally expressed fluorophore. Viral fluorescence is first observed as a single cell, and gradually spreads over time (i.e. Figure 3). The virus should spread within a few hours.

2. Passage and Concentration of VSV

1. Split TVA800 cells into media containing DMEM + 10% FBS such that you will have four 10-cm plates at ~80% confluency the next day. 293T-based cells work well due to their high transfectability, and the use of TVA-expressing cells enhances the speed at which the cells in the dish become infected. Other highly transfectable cell lines may serve the purpose as well.
2. At 80% confluency, change media to DMEM only, then transfect the cells with 5 μg of plasmid encoding the G protein that you want to use (i.e. pCAG RABV-G) per plate. We use PEI, but FUGENE/Lipofectamine work as well.
3. Wait one day after the transfection for glycoprotein expression/surface accumulation. Change the media (5 ml/plate) to DMEM + 10% FBS, then infect with rVSV(A/RG) virus at an multiplicity of infection (MOI) of 0.01 < x < 0.1.
4. Check the amount of infection 24 hr later. You should see infected cells (identified by GFP fluorescence), some with surrounding patches of infected cells (i.e. Figure 3). Typically it’s only worth collecting the supernatant if you see >50% of cells infected, otherwise the viral titers obtained from concentration will be suboptimal.
5. If > 50%, collect the 5 ml of media/plate, and replace with 5 ml of fresh media. If too few cells are infected, wait for another 12-24 hr, and check again. Freeze the collected supernatants at -80 °C.
6. Collect every 24 hr thereafter for 3 days, for a total of 4 days.
7. Altogether, if you started with 4 plates, you should have 20 ml of media from each day – so 80 ml total, or 2 ultracentrifuge tubes worth of supernatant. Thaw the supernatants at 37 °C and filter over 0.45 μm filters on ice. Aliquot 36 ml of virus into Beckman centrifuge tubes.
8. Concentrate the supernatant (21,000K in an SW28 rotor (=80,000 x g) for 90 min) at 4 °C. Decant the supernatant into a beaker containing 10% bleach, and while the tube is inverted, use an aspirator to remove as much media from the side of the tube as possible.
9. While covered. shake the viral tubes at 4 °C for 1 hr. A standard shaker at about 120 rpm is sufficient.
10. Gently triturate pellet in the remaining media by gently pipetting up and down 30 times. Be careful not to introduce air bubbles. The remaining media volume should be about 30 μl. Aliquot this volume into multiple tubes so as to prevent multiple freeze-thaw cycles for any particular stock, as this can result in a decrease in viral titer. These aliquots can be frozen at -80 °C.
11. Titer the virus. Titering at about 2 days post-infection is optimal. Initial infection can be observed in a matter of hours, but you get an underrepresentation of titer if you wait only 1 day. In order to obtain the viral titer, perform a limiting dilution experiment of the concentrated virus onto the appropriate cell line (293T cells work well) in a 24-well plate, diluting the virus 10-fold from 1 to 1 : 1,000,000. The titers we get are typically in the range of 10⁸-10¹⁰ focus forming units (ffu)/ml.

3. Virus Injection

1. We use mice, typically between 6-10 weeks of age, for these experiments. Any age after which circuitry is established would suffice. We will choose the transmission from retinal ganglion cells (RGCs) to starburst amacrine cells (SACs) as an example. This experiment, as diagramed in Figure 2, requires a triple transgenic animal and only a single injection of virus. (All procedures shown were approved by the IACUC at Harvard Medical School, and were in accordance with institutional guidelines).
2. First, the proper animals need to be generated. The goal is to have TVA expressed in the cell type that one wishes to map into, by means of a virus (i.e. adeno-associated virus, AAV), or by a transgenic allele. The cell type specificity is usually obtained by Cre expression. Here, we also crossed in a conditional expression TVA allele⁷ to a Choline Acetyltransferase (ChAT)-Cre allele¹³, such that TVA is expressed in all cells with a Cre expression history (the SACs). We also crossed a conditional expression tdTomato allele (R26TdT) for visualization of TVA-expressing cells.
3. The coordinates for the injection location need to be identified. These coordinates of interest can be found in a brain atlas, i.e. the Franklin and Paxinos mouse brain atlas¹⁴. These coordinates need to be noted relative to a given landmark on the mouse skull. We use bregma, but any coordinate system is sufficient.
4. Prepare the stereotaxic apparatus. Turn on the microinjector controller, and move the plunger and electrode holder into place. Then back-fill the injection needle with mineral oil to provide an interface with the virus. Put the needle then onto the plunger, making sure that no air bubbles remain in the needle.
5. Thaw the virus, and place on ice to prevent a drop in viral titer. Set the microinjector to “withdraw” and move the tube of virus such that that is contacting the capillary on the microinjector. Withdraw the necessary amount of rVSV(A/RG) pseudotyped with RABV-G for the experiment.
6. Animals are given a pre-operative dose of buprenorphine (0.05-0.1 mg/kg) before the surgery begins. For anaesthetizing the animal, either isoflurane inhalation, or an intraperitoneal injection of a ketamine (40-80 mg/kg) and xylazine (5-10 mg/kg) mixture is sufficient. Choice of anesthetic is dependent on the user. A toe pinch is then performed to ensure that animals are fully anesthetized; if the animal does not respond, you can proceed with procedure. Be sure to obtain proper licenses before using these drugs.
7. Animals are placed on a heated pad, which is propped on a movable stand for positioning the mouse. The animal remains there throughout the duration of the surgical procedure. Apply eye ointment to prevent drying of the cornea while the animal is anesthetized.
8. The mouse’s head then needs to be stabilized in the stereotaxic apparatus. Use the ear bars on the stereotaxic apparatus to ensure that the head is thoroughly secured, such that the head is parallel to the ground, and does not rotate laterally. Once this is completed, adjust the bite bar to help stabilize the head.
9. The animal’s fur is shaved in the region of incision on top of the head. This area is then washed three times with ethanol, followed by three times with iodine. An incision is then made in the skin with a scalpel, revealing the skull.
10. Once the mouse and injection setup are prepared, bregma needs to be located. This is the area in the center of the head that the sagittal and two coronal sutures meet. Once this area is located, the dials on the stereotaxic apparatus can be used to adjust the microinjector to the proper coordinates. Here, we inject the lateral geniculate nucleus (LGN), using coordinates A/P -2.46 from bregma, L/M 2, D/V 2.75.
11. Once the coordinates have been dialed in, the drill must be assembled. Place the drill bit into the drill, then turn on the drill. A small hole should be drilled into the site identified as the injection site. Be very gentle with the drill in order to not cause damage to the brain parenchyma. When the bone is very thin, blood vessels on the dura become clear. At this point, carefully perforate the edges of the craniotomy with a small (30 gauge) needle and a fine (#5) forceps to remove the remaining bone.
12. Adjust the capillary to a position just dorsal of the brain surface, where the hole was just drilled. The needle should be sharp enough to penetrate the dura mater with ease. Then lower the needle to the desired depth, and begin injection. We inject virus at a rate of 100 nl/min.
13. The injection procedure to this point takes roughly ten minutes. Following injection, the needle remains in the brain for about seven minutes. This is crucial, as it permits diffusion of virus from the injection site. Rapidly removing the needle form the injection site results in a reduced infection efficiency at the site of injection, and infection along the needle tract.
14. The needle is very slowly raised from the injection site, over a span of about two minutes. This is again intended to reduce infection along the needle tract.
15. Once removed, the skin is then sutured. Animals are monitored continuously until they recover from anesthesia. Buprenorphine (0.05-0.1 mg/kg) is administered every 12 hr for 2 days.

4. Harvesting Tissue/Tissue Preparation

1. For harvesting the tissue of interest, the optimal time depends on the goals of the experiment. Generally, VSV transsynaptic spread can first be observed by less than 24 hr post-injection. However, waiting longer times will result in more spread.
2. To harvest the retina, animals are first euthanized using carbon dioxide inhalation and cervical dislocation is performed. The eyeball is then removed, and placed into a tube containing 4% formaldehyde in PBS for 1 hr at room temperature.
3. The eyeball is then placed into a Petri dish with PBS. The retina is then dissected away from other tissues.
4. For whole mount analysis, the retinae are cut so as to lay flat, placed onto a glass slide, then mounted with prolong gold mounting media and covered with a zero-thickness glass coverslip. Silicone spacers are placed between the slide and coverslip to minimize compression forces on the retina. For section analysis, the retina is placed in 30% sucrose in PBS until the retina submerges. It is then placed into a mixture of 50% OCT and 50% 30% sucrose solution for 3 hr. It is then flash frozen, and kept at -80 °C until ready to cut.

5. Representative Results

The virus that is rescued from cDNA should be able to infect TVA800 cells, but not 293T cells (Figure 3). Supplying the RABV-G permits the infection of multiple cell types, including 293T. However, since the A/RG genome is encoded in the genome, spread among cells occurs at a much higher rate in TVA-expressing cells.

Once the virus is concentrated, typical titers range between 10⁸ ffu/ml and 10¹⁰ ffu/ml. These titers are adequate for use in vivo.

During LGN injection into the mouse, we do not distinguish dorsal LGN (dLGN) from ventral LGN (vLGN), as both typically become infected. Therefore, multiple RGC types become labeled (Figure 4), including those that project to the vLGN, such as melanopsin RGCs (Figure 4D) and ON-DSGCs (Figure 4E), and those that project to the dLGN, such as the small-arbor RGCs (Figure 4C) and ON-OFF-DSGCs (Figure 4F). Although more than one type of RGC transmitted virus to SACs, as reported elsewhere (Beier et al., in review), here we focus only on the well-studied connections of ON-OFF-DSGCs to SACs.

When we inject mice of the genotype cTVA(+)/ChAT-Cre(-), where no TVA should be expressed, only RGCs are labeled (i.e. Figure 4). These are due to initial uptake of the RABV-G pseudotyped virus by the RGCs, and no spread occurs. No RGCs are infected from an LGN infection of rVSV(A/RG) virus not pseudotyped with RABV-G, due to an inability of these virions to transverse the long axons of these RGCs. However, when injecting cTVA(+)/ChATCre(+) (and R26TdT(+)) mice into the LGN, viral spread does occur, only to red cells, which express the Cre reporter (i.e. Figure 5). These cells also co-label with the anti-ChAT antibody, and stratify only in the two ChAT laminae, confirming their identity as SACs (Figures 5B’, C’). The number of virally labeled SACs per DSGC ranged from one to nine.

Figure 1. Our retrograde transsynaptic tracing system compared to the originally developed method. (A) (i) In the method developed by Wickersham and colleagues, “starter cells” are transfected with three genes: the TVA receptor, used to permit infection specifically of transfected cells; the RABV-G, used to complement the RABV, which itself had the G gene deleted; and a red fluorescent protein to identify transfected cells. (ii) A RABV pseudotyped with the A/RG protein infects TVA-expressing cells, making them yellow. (iii) Retrograde transsynaptic transmission occurs to upstream neurons. (B) (i) In our method, TVA-expressing cells are defined genetically, and are labeled with a conditional red fluorescent protein. (ii) The “starter cells” are those infected by a VSV encoding the A/RG gene in the viral genome. These starter cells do not express the TVA receptor. (iii) Retrograde transsynaptic transmission occurs to TVA-expressing neurons only if these cells provide synaptic input onto the starter neurons. Click here to view larger figure.

Figure 2. Schematic of the experimental procedure. (A) First, the virus is rescued from cDNA. It is then passaged and pseudotyped with RABV-G, and concentrated and titered. This virus is then injected into the brain, where it is allowed to incubate for the desired period of time. After this time, the eye is harvested, and the retina dissected and analyzed. (B) Schematic of pseudotyping the virus with RABV-G. This is necessary for infection of retinal ganglion cells from a brain injection. The RABV-G gene is first transfected into tissue culture cells expressing the TVA receptor. These cells are then infected with the rVSV(A/RG) virus. The virions released will have RABV-G in the viral envelope, but will not have the gene for RABV-G in the viral genome. (C) A schematic of the mouse crosses necessary for triple transgenic animals. Conditional TVA and tdTomato alleles are crossed to a Cre driver of choice, such that both TVA and tdTomato are expressed only in cells with a Cre expression history. In this example, we used ChAT-Cre. (D) A schematic of the retinal infection in order to trace DSGC-SAC circuits. The ChAT cells are made to express TVA and tdTomato, as shown in (C). RGCs are infected from an LGN infection of the virus produced in (B). As the virus expresses GFP, these RGCs will be green. The virus will then spread only to TVA-expressing ChAT amacrine cells if they are connected to the labeled RGC. These cells will be both red and green, or yellow. Click here to view larger figure.

Figure 3. Behavior of rVSV(A/RG) virus on 293T and TVA800 cells. These cells were infected with a low titer rVSV(A/RG) pseudotyped with RABV-G. The virus spreads between TVA-expressing cells in a matter of hours, as shown at 6 hours post infection (HPI), 1 and 2 days post infection (DPI). However, the spread on 293T cells, which do not express the TVA receptor, though it does occur, happens much slower. Scale bar = 100 μm.

Figure 4. Representative infections of RGCs of non-TVA expressing mice. Animals were injected into the LGN with the rVSV(A/RG) virus pseudotyped with RABV-G, and tissue harvested 2 dpi. (A) Sparse (RGCs indicated by yellow arrowheads) or (B) dense infections can be obtained, depending on the titer of virus and quality of injection. Many different types of RGCs can be identified based on morphology, including (C) small arbor RGCs, (D) type 1 melanopsin RGCs, (E) ON-DSGCs, and (F) ON-OFF-DSGCs, among others. Scale bars = 50 μm.

Figure 5. rVSV(A/RG) transmission from ON-OFF-DSGCs to SACs follows the expected pattern. (A) ON-OFF-DSGCs (white arrowhead with green fill) transmit virus to multiple SACs (yellow arrowheads). (A’). All green cells that are not the DSGC co-label with the Cre reporter, indicating that they are TVA-expressing SACs. (B-C) The DSGC (white arrowhead with green fill) and SACs (yellow arrowheads) stratify in the appropriate ChAT layers of the retinal inner plexiform layer (IPL). The cells to which the virus transmits include both ON and OFF-SACs. Numbers in panels B’ and C’ indicate the IPL laminae. The location of the DSGC soma is only indicated by the arrowhead but not shown in panel C to highlight the SACs. Scale bars = 50 μm.