Generating an Ex Vivo Ovine Wounded Skin Model Infected with a Bacterial Pathogen

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Sterilization Disinfect forceps prior to collection of the heads by taking clean forceps and performing dry heat sterilization in an oven at 200 °C for 1 h. Autoclave all glassware at 121 °C for 15 min before use. Carry out all described work within a microbiology class 2 cabinet. Prepare all reagent…