Intracranial Cannular Implantation in a Mouse to Deliver Therapeutic Agents to Brain Tumors
Published: May 31, 2024
Abstract
Source: Harvey, K. et al., Intracranial Cannula Implantation for Serial Locoregional Chimeric Antigen Receptor (CAR) T Cell Infusions in Mice. J. Vis. Exp. (2023)
This video presents a procedure for intracranial cannula implantation in a brain tumor mouse model, to facilitate the administration of an immunotherapeutic agent. The delivery of glypican-2-specific chimeric antigen receptor T cells in the tumor microenvironment triggers T cell activation, resulting in tumor lysis and tumor regression.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Preparing the mouse for surgery Anesthetize the mouse in an induction chamber with isoflurane (2-4%) at an oxygen flow rate of 1 L/min until an adequate plane of anesthesia is reached (approximately 5 min). Weigh the mouse using a scale to the nearest 0.1 g and administer subcutaneous slow-release (SR) bupreno…
Representative Results
Figure 1: Guide cannula with projection dummy and treatment cannulas. (A) Guide cannula with 0.5 mm projection and 2 mm projection dummy cannulas in place. (B) Guide cannula with 0.5 mm projection and 2 mm projection treatment cannulas in place.
Divulgations
The authors have nothing to disclose.
Materials
| 18 G needles | BD | 511097 | 1 1/2 inch metal hub |
| Acrylic resin liquid | Lang Dental | B1323 | |
| Acrylic resin powder | Lang Dental | B1323 | |
| Alcohol wipes | BD | 326895 | |
| Centrifuge 5240 | Eppendorf | 5420000040 | Centrifuge |
| Cotton tipped swabs | Puritan | 826-WC | Handle Width = 2.11 mm (0.083), Head Width = 1.27 mm (0.050), Handle Length = 147.62 mm (5.812), Overall Length = 152.4 mm (6), Head Length = 12.7 mm (0.500) |
| Drill bit holder | P1 Technologies | DH-1 | Drill bit holder for D56-D70 |
| Drill bit | P1 Technologies | D58 | 1.07 mm |
| Dummy cannula | P1 Technologies | C315DCS-5/SPC | Configuration: Small cap; Length: Cut 5.00 mm below pedestal; Projection: 0.50 mm |
| Flat tip screwdriver | P1 Technologies | SD-80 | Screwdriver |
| Graefe forceps | Fine Science Tools | 11051-10 | Forceps |
| Guide cannula | P1 Technologies | C315GS-5/SPC | Configuration: 5.00 mm pedestal height; Length: Cut 5.00 mm below pedestal |
| Hemostatic cotton pellets with racemic epinephrine | Pascal | 1151602 | |
| MOXI Z Mini automated cell counter Kit | Moxi | MXZ001 | Cell counter |
| NOD scid gamma (NSG) mice | Jackson Laboratory | 5557 | 6 to 12-week-old males and females |
| Pasteur pipet | VWR | 14673-043 | |
| PKG tubing | P1 Technologies | C313CT | Diameter: 0.58 mm x 1.27 mm |
| Porcelain 12 well plate | Flinn Scientific | AP6064 | |
| Povidone iodine | Medline | MDS093943 | |
| Scalpel | World Precision Instrument | 50-822-457 | Disposable Scalpel, no.10, sterile, 10/box, Plastic Handle with 6" Ruler |
| Screws | P1 Technologies | 0-80 X 3/32 | 2.4 mm |
| Stereotaxic Frame | David Kopf Instruments | 940 | Model 940 Small Animal Stereotaxic Instrument with Digital Display Console |
| Student fine scissors | Fine Science Tools | 91460-12 | Scissors |
| Treatment cannula | P1 Technologies | C315IS-5/SPC | 33GA; Configuration: Standard internal; Length: Cut 5.00 mm below pedestal; Projection: 0.50 mm |
| Treatment syringes | Hamilton | 87908 | 5 µL, Model 75 Cemented Needle Special (SN) Syringe, 75SN/22/0.5"/PT3 |
| Vactrap XL | Foxx Life Sciences | 305-4401-FLS | Vacuum System |
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Citer Cet Article
Intracranial Cannular Implantation in a Mouse to Deliver Therapeutic Agents to Brain Tumors. J. Vis. Exp. (Pending Publication), e22232, doi: (2024).