Establishing a Mouse Model of Pleural Disseminated Lung Cancer
Published: October 31, 2023
Abstract
Source: Yasui, H., et al. Near Infrared Photoimmunotherapy for Mouse Models of Pleural Dissemination. J. Vis. Exp. (2021)
This video demonstrates a method to generate a mouse model of pleural dissemination by injecting lung cancer cell suspension into the thoracic cavity of an immunodeficient mouse.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Generation of a pleural dissemination model Prepare luciferase-expressing target cells and suspend 1.0 × 106 target cells in 100 µL of phosphate-buffered saline (PBS) NOTE: Intrathoracic cancer cells such as lung cancer and MPM are suitable as target cells. Luciferase-express…
Representative Results
Figure 1: Easy hand-made device for cell transplantation. Attach the stopper made with polystyrene foam to the 30G needle so that the tip remains at 5 mm. The tip of the needle should be bent to avoid pneumothorax. Figure 2: Injection of target cells in…
Divulgations
The authors have nothing to disclose.
Materials
| 1mL syringe | TERUMO | SS-01T | for mice experiment |
| 30G needle | Nipro | 1907613 | for mice experiment |
| BALB/cSlc-nu/nu | Japan SLC | ||
| D-Luciferin (potassium salt) | Cayman Chemical | 14681 | for bioluminescence imaging and DLIT |
| Isoflurane | Wako | 095-06573 | for mice anesthesia |
| 0.25w/v% Trypsin-1mmol/l EDTA 4Na Solution with Phenol Red | Wako | 209-016941 | for cell culture |
| RPMI-1640 with L-glutamine and Phenol Red | Wako | 189-02025 | for cell culture |
Tags
Citer Cet Article
Establishing a Mouse Model of Pleural Disseminated Lung Cancer. J. Vis. Exp. (Pending Publication), e21762, doi: (2023).