Subcutaneous Injection of Bacterial Antigen to Induce Systemic Inflammation in a Murine Model

Published: September 29, 2023

Abstract

Source: John, S. et al., Primed Mycobacterial Uveitis (PMU) as a Model for Post-Infectious Uveitis. J. Vis. Exp. (2021)

This video demonstrates the induction of systemic inflammation in an anesthetized mouse. The injection of mycobacterial antigen-adjuvant depots triggers cytokine release and localized inflammation. Lymphatic vessels open, enabling immune cell entry into lymph nodes and activation of T cells, which release pro-inflammatory cytokines, leading to widespread inflammation.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Antigen preparation for subcutaneous injection Perform all procedures in this section inside a chemical fume hood to prevent inhalation or skin contact with the Mycobacterium tuberculosis, Mtb H37Ra powder. Handle according to your institutional policies for Complete Freund's Adjuvant (typically BSL-1). Thi…

Divulgations

The authors have nothing to disclose.

Materials

Freund's Incomplete Adjuvant BD Difco, NJ, USA 263910
H37Ra lyophilized Mycobacteria extract BD Difco, NJ, USA 231141
Insulin needle Exel International, USA 26029 1 mL
Isoflurane
PBS Gibco 14190
RM2255 Leica Biosystems, IL,USA Tissue Sectioning
TB Syringe Becton, Dickinson and Company, NJ, USA 309602 1 mL
Tylenol Johnson & Johnson Consumer Inc, PA, USA NDC 50580-614-01 Acetaminophen

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Subcutaneous Injection of Bacterial Antigen to Induce Systemic Inflammation in a Murine Model. J. Vis. Exp. (Pending Publication), e21703, doi: (2023).

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