Subcutaneous Injection of Bacterial Antigen to Induce Systemic Inflammation in a Murine Model
Published: September 29, 2023
Abstract
Source: John, S. et al., Primed Mycobacterial Uveitis (PMU) as a Model for Post-Infectious Uveitis. J. Vis. Exp. (2021)
This video demonstrates the induction of systemic inflammation in an anesthetized mouse. The injection of mycobacterial antigen-adjuvant depots triggers cytokine release and localized inflammation. Lymphatic vessels open, enabling immune cell entry into lymph nodes and activation of T cells, which release pro-inflammatory cytokines, leading to widespread inflammation.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Antigen preparation for subcutaneous injection Perform all procedures in this section inside a chemical fume hood to prevent inhalation or skin contact with the Mycobacterium tuberculosis, Mtb H37Ra powder. Handle according to your institutional policies for Complete Freund's Adjuvant (typically BSL-1). Thi…
Divulgations
The authors have nothing to disclose.
Materials
| Freund's Incomplete Adjuvant | BD Difco, NJ, USA | 263910 | |
| H37Ra lyophilized Mycobacteria extract | BD Difco, NJ, USA | 231141 | |
| Insulin needle | Exel International, USA | 26029 | 1 mL |
| Isoflurane | |||
| PBS | Gibco | 14190 | |
| RM2255 | Leica Biosystems, IL,USA | Tissue Sectioning | |
| TB Syringe | Becton, Dickinson and Company, NJ, USA | 309602 | 1 mL |
| Tylenol | Johnson & Johnson Consumer Inc, PA, USA | NDC 50580-614-01 | Acetaminophen |
Tags
Citer Cet Article
Subcutaneous Injection of Bacterial Antigen to Induce Systemic Inflammation in a Murine Model. J. Vis. Exp. (Pending Publication), e21703, doi: (2023).