Assessment of Chemical-Induced Colitis via Immunohistochemical Analysis of Colon Tissue Sections
Published: September 29, 2023
Abstract
Source: Du, Y. W. et al., Effects of Taste Signaling Protein Abolishment on Gut Inflammation in an Inflammatory Bowel Disease Mouse Model. J. Vis. Exp. (2018)
This video demonstrates the assessment of chemical-induced colitis via immunohistochemical staining of mouse colon tissue. In a stained tissue section, the presence of a large number of immune-responsive cells in the damaged tissue represents the infiltration of immune cells during colitis.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Preparation of Mice and DSS Keep the knockout (α-gustducin-/-) mice and age-, gender-, and body weight-matched wild-type control (α-gustducin+/+) C57BL/6 mice individually in clean cages. NOTE: The knockout mice have been backcrossed with C57BL/6 mice for over 20 g…
Divulgations
The authors have nothing to disclose.
Materials
| Antibody | |||
| CD45 | BD Biosciences | 550539 | |
| CD3 | BD Biosciences | 555273 | |
| B220 | BD Biosciences | 550286 | |
| CD11b | BD Biosciences | 550282 | |
| Ly6G | BD Biosciences | 551459 | |
| Reagent | |||
| Dextran Sulfate Sodium Salt (DSS) | MP Biomedicals | 2160110 | |
| Streptavidin-HRP complex | BD Pharmingen | 551011 | |
| Phosphate-Buffered Saline (PBS) | Sangon Biotech | B548117 | |
| BD 10 ml Syringe | BD Biosciences | 309604 | |
| Instruments and equipment | |||
| Balance | |||
| Scissors | |||
| Forceps | |||
| Staining jars | |||
| Software | |||
| Imag-Pro Plus | Media Cybernetics, Inc. |
Tags
Citer Cet Article
Assessment of Chemical-Induced Colitis via Immunohistochemical Analysis of Colon Tissue Sections. J. Vis. Exp. (Pending Publication), e21644, doi: (2023).