Impedance-Based Cellular Assay to Measure Multiplicity of Infection of Influenza Virus
Published: April 30, 2023
Abstract
Source: Labadie, T., et al., Monitoring Influenza Virus Survival Outside the Host Using Real-Time Cell Analysis. J. Vis. Exp. (2021)
In this video, we discuss the correlation between the CIT50 and the multiplicity of viral infections. The virus-infected cells display cytopathic effects, causing their death and detachment from the gold microelectrode, which in turn, reduces the impedance.
Protocol
1. Correlation between CIT50 values and multiplicity of infection Add 100 µL of sterile 1x MEM culture medium in each well of the E-plate. Insert the E-plate into the cradle pocket of the instrument at 35°C. Measure the background as described: Open the software. In "Default experiment pattern setup", choose the selected cradle(s) and double-click on the top page, then enter the name of the experiment. Click "Layout"…
Divulgations
The authors have nothing to disclose.
Materials
| E-Plate 16 (6 plates) | ACEA Biosciences, Inc | 5469830001 | E-plates are available in different packaging |
| MEM 1X | Life technologies (gibco) | 31095029 | |
| TPCK-Trypsin | Worthington | LS003740 | |
| xCELLigence Real-Time Cell Analysis Instrument S16 | ACEA Biosciences, Inc | 380601310 | The xCELLigence RTCA S16 instruments are available in different formats (16-well, 96-well, single or multi-plate) |
Tags
Citer Cet Article
Impedance-Based Cellular Assay to Measure Multiplicity of Infection of Influenza Virus. J. Vis. Exp. (Pending Publication), e21324, doi: (2023).