Deneysel metastaz ve CTL Kabul edilmiş Transfer İmmünoterapi Fare Modeli
Summary
Tümör hücrelerinin T hücre etkileşimi analizi için deneysel bir akciğer metastazı ve CTL immünoterapi fare modeli<em> In vivo</em>.
Abstract
Deneysel metastaz fare modeli basit ve fizyolojik ilgili henüz metastaz modeli. Tümör hücrelerinin fare kuyruğu damarlar içine (iv) damar içine enjekte ve tümör hücre spontan metastaz son adımları benzeyen, böylece, akciğerlerde kolonize: distal organlarda kan dolaşımı, ekstravazasyonu ve sömürgeciliğin yaşam. Metastatik tümör distal organ yılında kurulan: tedavilerin hedef genellikle metastaz bitiş noktası bu yana tedavi edici bir açıdan bakıldığında, deneysel metastaz modeli en basit ve ideal bir model. Bu modelde, tümör hücrelerinin fare kuyruğu damarlar içine iv enjekte edilir ve akciğerlerde kolonize ve büyümeye izin. Tümör spesifik CTL sonra iv metastaz taşıyan fare içine enjekte edilir. Akciğer metastazları sayısını ve boyutunu enjekte edilecek tümör hücrelerinin sayısı ve tümör büyümesini zaman kontrol edilebilir. Bu nedenle, çeşitli aşamalarında metastaz, geniş metastaz minimal metastaz modellenmiş olabilir. Akciğer metastazı, böylece daha kolay görsel gözlem ve miktar sağlayarak, mürekkep ile enflasyon analiz edilir.
Protocol
Divulgations
The authors have nothing to disclose.
Acknowledgements
Ulusal Sağlık Enstitüleri (KL için CA133085) ve Amerikan Kanser Derneği (KL RSG-09-209-01-TBG) hibe ile desteklenir.
Materials
Solutions:
India Ink Solution (17):
- Pour 150 ml of distilled water into a 250 ml flask.
- Add 4 drops ammonium hydroxide to the distilled water.
- Add 30 ml India Ink stock (i.e. Sanford Black Magic Waterproof Drawing Ink 4465 Item 44011) to the ammonia and water mixture.
- Top off with distilled water to a volume of 200 ml. Solution is ready for injection.
Fekete’s Solution (17):
Fekete’s solution is used to bleach India ink-inflated tumor-bearing lungs to distinguish white tumor nodules from the black background of normal tissues.
- Add 350ml 95% EtOH to 1L glass bottle.
- Add 150ml distilled water
- Add 50ml formaldehyde
- Add 25ml glacial acidic acid
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