Prenatal Mouse Embryos Retrieval: A Procedure to Harvest Embryos from Pregnant Mouse
Published: April 30, 2023
Abstract
Source: Yun, K. Assessing Urinary Tract Junction Obstruction Defects by Methylene Blue Dye Injection. J. Vis. Exp. (2017)
This video demonstrates the retrieval of embryos from a pregnant mouse. The recovered perinatal embryos can be used as model systems for studying mammalian development.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Dissection of Prenatal Embryos Clean dissecting scissors and forceps with 70% ethanol. To collect perinatal embryos at E18.5 or E19.5, euthanize pregnant mice first using CO2 inhalation and then perform cervical dislocation according to ethical guidelines. NOTE: The preg…
Divulgations
The authors have nothing to disclose.
Materials
| Delicate Operating Scissors | Roboz Surgical Instrument Co. | RS-6702 | |
| Micro Dissecting Forceps | Roboz Surgical Instrument Co. | RS-5135 | |
| Dumont Tweezers; Pattern #5 | Roboz Surgical Instrument Co. | RS-5045 | |
| Quality Biological Inc. NORMAL SALINE – 500ML | Fisherscientific | 50-983-204 |
Tags
Citer Cet Article
Prenatal Mouse Embryos Retrieval: A Procedure to Harvest Embryos from Pregnant Mouse. J. Vis. Exp. (Pending Publication), e20754, doi: (2023).