Transverse Aortic Arch Banding in Mouse Model: A Minimally Invasive Technique in Mice for Induction of High Pressure in the Left Ventricle

Published: April 30, 2023

Abstract

Source: Zaw, A. M. et al. Minimally Invasive Transverse Aortic Constriction in Mice. J. Vis. Exp. (2017)

In this video, we demonstrate an experimental protocol for minimally invasive transverse aortic constriction in mice to examine pressure overload-induced heart failure.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Operation Site Preparation Disinfect the operation site with 75% isopropyl alcohol. Adjust the heating pad temperature to 37 °C to avoid changes in heart and respiratory rates of the subject animal. Use surgical instruments sterilized in a gravity displacement sterilizer at 121 °C fo…

Representative Results

Figure 1: Process of forming the desired needle hooks by bending a 1-in, 30-gauge needle tip. (A) The original 1-in, 30-gauge needle tip. (B) Grasp the needle tip with the needle holder and bend approximately halfway along its length. Bend the needle into a loop, using the needle holder and the hands, as necessary, to form the loop pictured in th…

Divulgations

The authors have nothing to disclose.

Materials

1 inch 30-gauge Needles BD, Franklin Lakes, NJ 07417, USA 305128 Curved as in the procedure and Figure 1
27-gauge Needle BD, Franklin Lakes, NJ 07417, USA 301629 Make blunt and smooth for spacer
6/0 Dafilon B. Braun, 34212 Melsungen, Hessen, Germany C0933066
6/0 Silkam B. Braun, 34212 Melsungen, Hessen, Germany C0762067
Sterile gloves A.R. Medicom, Inc (Asia), Hong Kong
Cotton Applicator Mannings, Quarry Bay, Hong Kong Local Shopping Center
Depilatory Cream Veet (Hong Kong), Kwun Tong, Kowloon, Hong Kong Local Shopping Center
Lexer-Baby Scissor FST, British Columbia V7H 0A6, Canada 14078-10
Curved Iris Forcep FST, British Columbia V7H 0A6, Canada 11065-07
Micro Olsen-Hegar Needle Holder WPI, Sarasota, FL 34240, USA 501989
Stereo Microscope WPI, Sarasota, FL 34240, USA PZMIII-BS
GenTeal Eye Gel Novartis, East Hanover, NJ 07936, USA Local Pharmacy
Heating Pad Kent Scientific Corporation, Torrington, CT 06790, USA DCT-20
Surgical tape Laboratory Animal Unit
Ketamine and Xylazine Laboratory Animal Unit
Betadine Laboratory Animal Unit
Buprenorphine Laboratory Animal Unit

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Transverse Aortic Arch Banding in Mouse Model: A Minimally Invasive Technique in Mice for Induction of High Pressure in the Left Ventricle. J. Vis. Exp. (Pending Publication), e20752, doi: (2023).

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