Rabbit Intraductal Injection: Localized Delivery of Solution of Interest into the Rabbit Mammary Gland
Published: April 30, 2023
Abstract
Source: Clark et. al., Intraductal Delivery to the Rabbit Mammary Gland. J. Vis. Exp. (2017).
In this video we describe a technique of administering a solution of interest into the mammary duct of a rabbit and confirm the delivery by high-resolution ultrasound imaging of contrast agents.
Protocol
1. Preoperative Preparation Record the body weight of each rabbit. As with all preclinical studies, monitor animal weights regularly to assess potential toxicity. Prior to anesthetizing the rabbit, fill a 50 mL conical tube with commercially available ultrasound gel and spin at 500 x g for 30 s; there should be no visible bubbles in the gel upon completion of the centrifugation. Administer glycopyrrolate subcutaneously at a dose of 0.1 mg/kg and acepromazine intramuscularly at a dose of 0….
Divulgations
The authors have nothing to disclose.
Materials
| MicroMarker non-targeted contrast reagent | VisualSonics | VS-11694 | |
| Luer Lock 1 mL Syringes | BD | 309628 | |
| Glycopyrrolate 0.2 mg/mL | Wedgewood Compounding Pharmacy | GLYCOP-INJ013VC | 6 month shelf life, supply may be limited. |
| Ketamine HCL 100 mg/mL | Animal Health International | 21250699 | http:// www.animalhealthinternational.com/ |
| Acepromazine 10 mg/mL | Animal Health International | 17640541 | |
| Xylazine 20 mg/mL | Animal Health International | 20101547 | |
| Hair Removing Cream | Veet | Sensitive skin solution. Available through local retailers. | |
| Blunt tip infusion needles | Sai Infusion Technology | B14-50 | http://www.sai-infusion.com/ collections/blunt-needles |
| Veterinary Pulse Oximeter | EdanUSA | VE-H100B | http://www.edanusa.com/Product/ VE-H100B-Veterinary-PulseOximeter.html |
| Warm Water Pump | Gaymar | TP700 | |
| Warm Water Blanket | Animal Health International | 21232696 | Maxi-Therm Lite Warming Pads |
Citer Cet Article
Rabbit Intraductal Injection: Localized Delivery of Solution of Interest into the Rabbit Mammary Gland. J. Vis. Exp. (Pending Publication), e20224, doi: (2023).