Intraductal Injection: Delivering Injection Mix into the Ducts of the Mouse Mammary Gland
Published: April 30, 2023
Abstract
Source: Xiang, D., et al. Modeling Breast Cancer via an Intraductal Injection of Cre-expressing Adenovirus into the Mouse Mammary Gland. J. Vis. Exp (2019).
This video describes an intraductal injection technique where we deliver injection mix into the ducts of mouse mammary glands.
Protocol
1. Intraductal injection Use aseptic techniques throughout the surgical procedure. Make an incision site on the skin at a length of ~1 cm between the two fourth inguinal MGs (Figure 1). Carefully separate the skin flap (with the MG) from the parietal peritoneum so as to visualize the mammary ductal tree. Carefully hold the nipple with Watchmaker’s forceps and remove the exterior nipple without cutting any nearby skin, using a micro-dissection scissor. Load …
Representative Results
Figure 1: Schematic diagram of the intraductal injection of Ad-Cre virus into an MG. (A) Incision site in the midline between the two fourth MGs. (B) Intraductal injection of Ad-Cre with a blue dye (for better visualization) into one of the fourth MGs. (C) Closing of the incision in the skin by wound clips
Divulgations
The authors have nothing to disclose.
Materials
| 33-gauge needle | Hamilton | 7803-05 | point style 3 blunt |
| 7mm Reflex Clip | Braintree Scientific | RF7 CS | |
| Adenovirus, Ad-K5-Cre | University of Iowa Viral Vector Core | Ad5-bk5-Cre (VVC-Berns-1547) | |
| Adenovirus, Ad-K8-Cre | University of Iowa Viral Vector Core | Ad5mK8-nlsCre | |
| Bromophenol Blue | Sigma-Aldrich | B0126-25G | |
| Hamilton syringe | Hamilton | 7636-01 | 0.025 mL |
| Micro-dissecting scissors | Pentair | 9M | Watchmaker's Forceps |
| Micro-dissecting tweezers | Dumont | M5 |
Citer Cet Article
Intraductal Injection: Delivering Injection Mix into the Ducts of the Mouse Mammary Gland. J. Vis. Exp. (Pending Publication), e20217, doi: (2023).