Heart Dissection: A Method to Observe Zebrafish Cardiac Development
Published: April 30, 2023
Abstract
Source: Singleman C. et. al., Heart Dissection in Larval, Juvenile and Adult Zebrafish, Danio rerio. J. Vis. Exp. (2011).
This video describes a technique of isolating the heart from a zebrafish at multiple developmental stages to study cardiac development, maturation, and ageing.
Protocol
1. Measuring fish and dissection Place single fish into petri dish half filled with fresh PBS and lay it on its right side. Measure length of fish from snout to base of tail (the caudal peduncle), not including tail fins, using a digital mm caliper (Figure 1). This is the standard length (SL). Each fish can be assigned a number/letter combination to allow an individual fish and the resulting dissected heart to be tracked for later analysis. Data, including parental stock n…
Representative Results
Figure 1. Hold the fish with forceps between the eyes and gills. Figure 2. A cardiac specific fluorescent transgenic marker such as Tg(myl7:GFP)twu34, shown here, can aid in visualizing hearts dissected from smaller fish. <img a…
Materials
| Forceps #55 | Fine Science Tools | 11295-51 | |
| Micro-scissors | Fine Science Tools | 15005-08 | |
| Pin holder | Fine Science Tools | 26016-12 | |
| Micro-needles | Fine Science Tools | 10130-10 | |
| Petri dishes | BD Biosciences | 351029 |
Citer Cet Article
Heart Dissection: A Method to Observe Zebrafish Cardiac Development. J. Vis. Exp. (Pending Publication), e20200, doi: (2023).