Intramuscular (IM) Injection: Compound Delivery into the Dorsal Muscle of an Adult Zebrafish
Published: April 30, 2023
Abstract
Source: Myllymäki, H., et al. Immunization of Adult Zebrafish for the Preclinical Screening of DNA-based Vaccines. J. Vis. Exp. (2018).
This video describes the technique of intramuscular injection for delivering compounds into the dorsal muscles of an adult zebrafish.
Protocol
1. Injection of the DNA Vaccine and Electroporation For immunizations according to this protocol, use healthy, 6 to 12 month-old adult zebrafish. Keep the fish in a flow-through system with a 14/10 h light/dark cycle, with a maximum of seven fish per 1 L of water. Prepare a 0.02% 3-aminobenzoic acid ethyl ester (tricaine) solution (pH 7) in tank water for anesthetizing the fish. Use a 10 cm Petri dish or something similar. Prepare a recovery tank by filling a 5 L beaker with 3 L of clean s…
Materials
| Phenol red sodium salt | Sigma-Aldrich | 114537-5G | |
| Tricaine (ethyl 3-aminobenzoate methanesulfonate salt) | Sigma | A5040-100g | anestesia and euthanasia solution |
| Tweezertrodes Electrodes (7 mm) Kits | BTX Harvard apparatus | BTX 450165 | tweezer type electrodes |
| Aluminosilicate capillaries | Harvard apparatus | 30-0108 | |
| Micromanipulator | Narishige | MA-153 | |
| Microscope | Olympus | ZS61 | |
| ECM Electro Square Porator | BTX Harvard apparatus | BTX ECM 830 | |
| Plastic spoon | |||
| Sponge | |||
| Styrofoam workbench |
Citer Cet Article
Intramuscular (IM) Injection: Compound Delivery into the Dorsal Muscle of an Adult Zebrafish. J. Vis. Exp. (Pending Publication), e20194, doi: (2023).