JOVE JOURNAL

Immunologie et infection

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Journal (Immunology and Infection)

Collection and Processing of Lymph Nodes from Large Animals for RNA Analysis: Preparing for Lymph Node Transcriptomic Studies of Large Animal Species

Catherine E. Vrentas¹, Paola M. Boggiatto¹, Robert G. Schaut¹, Steven C. Olsen¹

¹National Animal Disease Center, Agricultural Research Service,U.S. Department of Agriculture

Journal (Immunology and Infection)

Evaluation of Blood Lactate and Plasma Insulin During High-intensity Exercise by Antecubital Vein Catheterization

Minas Nalbandian¹, Zsolt Radak², Masaki Takeda³

¹Graduate School of medicine,Kyoto University, ²Research Institute of Sports Science,University of Physical Education, ³Graduate School of Sports and Health Science,Doshisha University

Journal (Immunology and Infection)

Using Terminal Transferase-mediated dUTP Nick End-labelling (TUNEL) and Caspase 3/7 Assays to Measure Epidermal Cell Death in Frogs with Chytridiomycosis

Laura A. Brannelly^1,2, Alexandra A. Roberts¹, Lee F. Skerratt¹, Lee Berger¹

¹One Health Research Group, College of Public Health, Medical and Veterinary Sciences,James Cook University, ²Department of Biological Sciences,University of Pittsburgh

Journal (Immunology and Infection)

Assessment of the Cytotoxic and Immunomodulatory Effects of Substances in Human Precision-cut Lung Slices

Vanessa Neuhaus*¹, Olga Danov*¹, Sebastian Konzok¹, Helena Obernolte¹, Susann Dehmel¹, Peter Braubach², Danny Jonigk², Hans-Gerd Fieguth³, Patrick Zardo⁴, Gregor Warnecke⁴, Christian Martin⁵, Armin Braun^1,6, Katherina Sewald¹

¹Fraunhofer Institute for Toxicology and Experimental Medicine (ITEM), German Center for Lung Research (DZL),Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Member of REBIRTH Cluster of Excellence, ²Institute for Pathology, Hannover Medical School, German Center for Lung Research (DZL),Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), ³Division of Thoracic and Vascular Surgery,Klinikum Region Hannover (KRH), ⁴Department of Cardiothoracic, Transplantation and Vascular Surgery (HTTG), Hannover Medical School, German Center for Lung Research (DZL),Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), ⁵Institute of Pharmacology and Toxicology,RWTH Aachen University, ⁶Institute for Immunology,Hannover Medical School

Journal (Immunology and Infection)

Mouse- and Human-derived Primary Gastric Epithelial Monolayer Culture for the Study of Regeneration

Emma Teal¹, Nina G. Steele², Jayati Chakrabarti¹, Loryn Holokai³, Yana Zavros¹

¹Department of Pharmacology and Systems Physiology,University of Cincinnati, ²Department of Cell & Developmental Biology,University of Michigan, ³Department of Molecular Genetics, Biochemistry & Microbiology,University of Cincinnati

Journal (Immunology and Infection)

Preparation of Murine Submandibular Salivary Gland for Upright Intravital Microscopy

Xenia Ficht*¹, Flavian Thelen*¹, Bettina Stolp*^1,2, Jens V. Stein¹

¹Theodor-Kocher Institute,University of Bern, ²Center for Infectious Diseases, Integrative Virology,University Clinic of Heidelberg

Journal (Immunology and Infection)

Bile Salt-induced Biofilm Formation in Enteric Pathogens: Techniques for Identification and Quantification

Kourtney P. Nickerson^1,2, Christina S. Faherty^1,2

¹Mucosal Immunology and Biology Research Center, Division of Pediatric Gastroenterology and Nutrition,Massachusetts General Hospital, ²Department of Pediatrics,Harvard Medical School

Journal (Immunology and Infection)

Replication of the Ordered, Nonredundant Library of Pseudomonas aeruginosa strain PA14 Transposon Insertion Mutants

Eliana Drenkard*¹, Rhianna M. Hibbler*¹, D. Alina Gutu², Alexander D. Eaton¹, Amy L. Silverio¹, Frederick M. Ausubel^2,3, Bryan P. Hurley^1,4, Lael M. Yonker^1,4

¹Department of Pediatrics, Mucosal Immunology and Biology Research Center,Massachusetts General Hospital, ²Department of Molecular Biology,Massachusetts General Hospital, ³Department of Genetics,Harvard Medical School, ⁴Department of Pediatrics,Harvard Medical School

Journal (Immunology and Infection)

Injections of Lipopolysaccharide into Mice to Mimic Entrance of Microbial-derived Products After Intestinal Barrier Breach

Katarina Radulovic¹, Rachel Mak’Anyengo¹, Berna Kaya¹, Anna Steinert¹, Jan Hendrik Niess^1,2

¹Department of Biomedicine,University of Basel, ²Division of Gastroenterology and Hepatology,University Hospital of Basel

Journal (Immunology and Infection)

Construction of Synthetic Phage Displayed Fab Library with Tailored Diversity

Ganggang Huang*¹, Zhenwei Zhong*¹, Shane Miersch^2,3, Sachdev S. Sidhu^1,2,3, Shin-chen Hou¹, Donghui Wu¹

¹Laboratory of Antibody Engineering, Shanghai Institute for Advanced Immunochemical Studies,ShanghaiTech University, ²Banting and Best Department of Medical Research, Terrence Donnelly Center for Cellular and Biomolecular Research,University of Toronto, ³Department of Molecular Genetics, Terrence Donnelly Center for Cellular and Biomolecular Research,University of Toronto

Journal (Immunology and Infection)

The Lambda Select cII Mutation Detection System

Ahmad Besaratinia¹, Stella Tommasi¹

¹Department of Preventive Medicine, USC Keck School of Medicine,University of Southern California

Journal (Immunology and Infection)

Spatial and Temporal Control of T Cell Activation Using a Photoactivatable Agonist

Elisa Sanchez^1,2, Morgan Huse¹

¹Immunology Program,Memorial Sloan-Kettering Cancer Center, ²Weill-Cornell Graduate School of Medical Sciences

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