JOVE JOURNAL

Génétique

Showing 205 - 216 in 413 videos.

ATAC-seq Assay with Low Mitochondrial DNA Contamination from Primary Human CD4+ T Lymphocytes

Hannah D. Rickner¹, Sheng-Yong Niu^1,2, Christine S. Cheng^1,3

¹Department of Biology,Boston University, ²Department of Computer Science and Engineering,University of California San Diego, ³Bioinformatics Program,Boston University

Journal (Genetics)

Isolating, Sequencing and Analyzing Extracellular MicroRNAs from Human Mesenchymal Stem Cells

Yan Yan*¹, Chi-Chih Chang*¹, Morten T. Venø^1,2, Colin R. Mothershead¹, Junyi Su¹, Jørgen Kjems^1,2

¹The Interdisciplinary Nanoscience Centre,Aarhus University, ²Department of Molecular Biology and Genetics,Aarhus University

Journal (Genetics)

A Deep-sequencing-assisted, Spontaneous Suppressor Screen in the Fission Yeast Schizosaccharomyces pombe

Bahjat F. Marayati^1,2, James B. Pease¹, Ke Zhang^1,2

¹Department of Biology,Wake Forest University, ²Center for Molecular Communication and Signaling,Wake Forest University

Journal (Genetics)

qKAT: Quantitative Semi-automated Typing of Killer-cell Immunoglobulin-like Receptor Genes

Jyothi Jayaraman^1,2,3,4, Vitalina Kirgizova¹, Da Di^1,5, Christopher Johnson^1,6, Wei Jiang^1,7, James A. Traherne¹

¹Department of Pathology,University of Cambridge, ²Department of Physiology, Development and Neuroscience,University of Cambridge, ³Department of Obstetrics and Gynaecology,University of Cambridge School of Medicine, NIHR Cambridge Biomedical Research Centre, ⁴Centre for Trophoblast Research,University of Cambridge, ⁵Department of Genetics & Evolution,University of Geneva, ⁶Royal Papworth Hospital, ⁷Department of Plant Sciences,University of Cambridge

Journal (Genetics)

Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR

Carl Robert Rankin¹, Janet Treger¹, Emmanuelle Faure-Kumar², Jihane Benhammou¹, Deborah Anisman-Posner³, Alex Edward Bollinger³, Charalabos Pothoulakis¹, David Miguel Padua¹

¹Vatche and Tamar Manoukian Division of Digestive Diseases, Department of Medicine,University of California, Los Angeles, ²Vatche and Tamar Manoukian Division of Digestive Diseases, Integrated Molecular Technologies (IMT) Core, Department of Medicine,University of California, Los Angeles, ³Division of Hematology and Oncology, Department of Medicine,University of California, Los Angeles

Journal (Genetics)

Using Sniper-Cas9 to Minimize Off-target Effects of CRISPR-Cas9 Without the Loss of On-target Activity Via Directed Evolution

Joonsun Lee*¹, Min-hee Jung*¹, Euihwan Jeong*¹, Jungjoon K. Lee¹

¹ToolGen

Journal (Genetics)

A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues

Ranjan K. Behera¹, Kevin D. Mlynek¹, Matthew S. Linz¹, Shaun R. Brinsmade¹

¹Department of Biology,Georgetown University

Journal (Genetics)

Preparation and Gene Modification of Nonhuman Primate Hematopoietic Stem and Progenitor Cells

Stefan Radtke¹, Anai M. Perez¹, Rasika Venkataraman¹, Sowmya Reddy¹, Kevin G. Haworth¹, Olivier Humbert¹, Hans-Peter Kiem^1,2,3, Christopher W. Peterson^1,2

¹Stem Cell and Gene Therapy Program,Fred Hutchinson Cancer Research Center, ²Department of Medicine,University of Washington, ³Department of Pathology,University of Washington

Journal (Genetics)

Enhanced Yeast One-hybrid Screens To Identify Transcription Factor Binding To Human DNA Sequences

Shaleen Shrestha*¹, Xing Liu*¹, Clarissa Stephanie Santoso*¹, Juan Ignacio Fuxman Bass^1,2

¹Department of Biology,Boston University, ²Bioinformatics Program,Boston University

Journal (Genetics)

Using Ustilago maydis as a Trojan Horse for In Situ Delivery of Maize Proteins

Isabell-Christin Fiedler¹, Arne Weiberg², Karina van der Linde¹

¹Department of Biology,Regensburg University, ²Department of Biology,Ludwig-Maximilians University of Munich

Journal (Genetics)

Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation

Hazuki Takahashi*¹, Harshita Sharma*¹, Piero Carninci^1,2

¹Laboratory for Transcriptome Technology,RIKEN Center for Integrative Medical Sciences, ²TransSINE Technologies Co. Ltd.

Journal (Genetics)

Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes

Jingxin Wang¹, John Hammond², Kristen A. Johnson¹

¹Calibr,The Scripps Research Institute, ²Department of Integrative Structural and Computational Biology,The Scripps Research Institute

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