Pfizer Worldwide Research and Development 1 article published in JoVE Cancer Research Multidimensional Coculture System to Model Lung Squamous Carcinoma Progression Shuang Chen1, Andreas Giannakou1, Jonathon Golas1, Kenneth G. Geles1 1Oncology R&D group, Pfizer Worldwide Research and Development An in vitro model system was developed to capture tissue architectural changes during lung squamous carcinoma (LUSC) progression in a 3-dimensional (3D) co-culture with cancer-associated fibroblasts (CAFs). This organoid system provides a unique platform to investigate the roles of diverse tumor cell-intrinsic and extrinsic changes that modulate the tumor phenotype.