University College Cork View Institution's Website 5 articles published in JoVE Biology Fluorescence Lifetime Macro Imager for Biomedical Applications Rajannya Sen1, Alexander V. Zhdanov1, Ciaran Devoy2, Mark Tangney2, Liisa M. Hirvonen3, Andrei Nomerotski4, Dmitri B. Papkovsky1 1School of Biochemistry and Cell Biology, University College Cork, 2Cancer Research@UCC, University College Cork, 3Centre for Microscopy, Characterisation and Analysis (CMCA), The University of Western Australia, 4Physics Department, Brookhaven National Laboratory This paper describes the use of a new, fast optical imager for the macroscopic photoluminescence lifetime imaging of long decay emitting samples. The integration, image acquisition, and analysis procedures are described, along with the preparation and characterization of the sensor materials for the imaging and the application of the imager in studying biological samples. Immunology and Infection Development of an Antigen-driven Colitis Model to Study Presentation of Antigens by Antigen Presenting Cells to T Cells Valerio Rossini1, Katarina Radulovic2, Christian U. Riedel3, Jan Hendrik Niess2 1APC Microbiome Institute, University College Cork, 2Division of Gastroenterology and Hepatology, University Hospital Basel, 3Institute of Microbiology and Biotechnology, University of Ulm In this antigen-driven colitis model, OT-II CD4+ T cells expressing a red fluorescent protein were adoptively transferred into RAG-/- mice that express a green fluorescent protein in mononuclear phagocytes (MPs). The hosts were challenged with Escherichia coli (E.coli) expressing the ovalbumin protein (OVA) fused to a cyan fluorescent protein (CFP). Immunology and Infection Bioluminescent Bacterial Imaging In Vivo Chwanrow K. Baban*1, Michelle Cronin*1, Ali R. Akin1, Anne O'Brien1, Xuefeng Gao1, Sabin Tabirca1, Kevin P. Francis1, Mark Tangney1 1Cork Cancer Research Centre, BioSciences Institute, University College Cork This article describes the administration of lux-tagged bacteria to mice and subsequent in vivo analysis using IVIS bioluminescence imaging. Medicine Ex Vivo Culture of Patient Tissue & Examination of Gene Delivery Simon Rajendran1, Slawomir Salwa1, Xuefeng Gao2, Sabin Tabirca2, Deirdre O'Hanlon2, Gerald C. O'Sullivan1, Mark Tangney1 1Cork Cancer Research Centre, Mercy University Hospital and Leslie C. Quick Jnr. Laboratory, University College Cork, 2Department of Computer Science, University College Cork This article describes the culture of patient tissue slices for gene delivery studies and subsequent analysis of gene expression using IVIS bioluminescence imaging. Medicine Murine Bioluminescent Hepatic Tumour Model Simon Rajendran1, Slawomir Salwa1, Xuefeng Gao2, Sabin Tabirca2, Deirdre O'Hanlon3, Gerald C. O'Sullivan1, Mark Tangney1 1Cork Cancer Research Centre, Mercy University Hospital and Leslie C. Quick Jnr. Laboratory, University College Cork, 2Department of Computer Science, University College Cork, 3South Infirmary Victoria University Hospital This article describes a procedure for the induction of orthotopic bioluminescent liver tumours in mice, and subsequent analysis of tumour growth confined to the liver using live whole body luminescence imaging.