The First Affiliated Hospital of Ningbo University 1 article published in JoVE Medicine Non-Intubated Video-Assisted Thoracoscopic Surgery Gao Bin*1,2, Luo Wenjun*3, Gui Yu2, Xu Zhipeng2, Zhu Binbin2, Yu Lina1 1Department of Anesthesia, Second Affiliated Hospital of Zhejiang University School of Medicine, 2Department of Anesthesia, The First Affiliated Hospital of Ningbo University, 3Health Science Center, Ningbo University Here, we present a non-intubated protocol for performing video-assisted thoracoscopic surgery with preserved autonomic breathing.