Encyclopedia Of Experiments

Gene Transfer Techniques

This collection features gene transfer methods, including viral vector-mediated transduction and non-viral vector-mediated physical and chemical transfection techniques to deliver genetic material in cultured cells and in vivo animal models at their embryonic and adult stages. Highlights include transfection utilizing hydrodynamics, microinjection, biolistics, nucleofection, and magnetofection techniques, and transduction via retroviral, adenoviral, nanoblade, and lentiviral systems.

Encyclopedia Of Experiments

Gene Transfer Techniques

Sections

Transfection

Transduction

Transfection

Transduction

Transfection

Bead Loading to Introduce Nucleic Acids Into Adherent Cultured Cells

A Technique to Load Fluorescent Protein-Encoding Plasmids Into Adherent Mammalian Cells

Mosquito Embryo Microinjection

A Technique to Deliver Exogenous DNA into Embryo Yolk of Anopheles Gambiae to Generate Germline Mutations

Image-Guided Robotic Cell Microinjection

An Automated High-Throughput Technique for the Precise Delivery of Fluorescently-Labeled Polysaccharides Into Single Neuronal Cells in Organotypic Brain Slice Cultures

Hydrodynamic Gene Delivery

A Technique for In Vivo Transfection of Exogenous DNA in Murine Hepatocytes via Tail Vein Injection

Agrobacterium-Mediated Genetic Transformation

A Method to Genetically Transform the Rice Genome via Genetically Engineered Agrobacterium tumefaciens

Electroporation-Mediated In Vitro Gene Delivery

An Electric Pulse Technique to Introduce Fluorescent Reporter Protein-Encoding Plasmids Into Cultured Cells

Nucleofection of Parasite Forms

An Electroporation-Based Transfection Method to Deliver Fluorescent Protein Encoding-Plasmids Into Sporozoite Nucleus

Biolistic-Mediated Gene Transfer

A Technique to Deliver Gene of Interest in Target Cells via a Biolistic Gene Gun

Magnetofection-Based Transfection In Vitro

A Magnetic Field-Assisted Technique to Deliver Plasmids Into Primary Mouse Neuronal Cells Using Magnetic Nanoparticles

Transduction

Recombinant Adeno Associated Virus Based Gene Delivery

A Technique for Transgene Delivery and Cardiac-Specific Expression in Rodent Model

Nanoblade-Based Delivery of Nucleic Acid Cargo

A Technique to Deliver Cas9-sgRNA Complex to Target Cells via Virus-Like Particles

Lentiviral Vector-Based Perivitelline Injection

A Method to Transduce the Gene of Interest Into Fertilized Single-Cell Mouse Oocytes

Laser-Assisted Lentiviral Gene Delivery

A Technique to Permeabilize Mouse Fertilized Eggs to Facilitate Lentiviral Gene Delivery

Retroviral Mediated Gene Transduction

A Gene Transfer Technique to Deliver a Transgene in Cultured Cells Using Engineered Retroviral Vectors