Isolation, Expansion, and Nucleofection of Neural Stem Cells from Adult Murine Subventricular Zone

Esteban Jim&#233;nez-Villalba; Laura L&#225;zaro-Carot; Carmen M. Mateos-Mart&#237;nez; Jennifer D&#237;az-Moncho; Daniel Samper-Llavador; Marta Igual-L&#243;pez; Jordi Planells; Sacri  R. Ferr&#243;n

doi:10.3791/66651

Journal

Neurosciences

Efficient Nucleofection of NSCs Isolated from the Murine Subventricular Zone

June 14, 2024

Author Spotlight: Improved Nucleofection for High-Efficiency Gene Delivery in Murine Subventricular Zone-Derived Neural Stem Cell Cultures

01:38 min

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345 Views

Murine Brain Extraction and Subventricular Zone (SVZ) Dissection for Isolation of Neural Stem Cells

02:08 min

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41 Views

Isolation and Seeding of Murine Subventricular Zone-Derived Neural Stem Cells (NSCs) for Neurosphere Formation

02:14 min

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22 Views

Expansion of Neurospheres Obtained from Murine Subventricular Zone-Derived Neural Stem Cells

01:41 min

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21 Views

Nucleofection of Neurosphere Cultures Obtained from Murine Subventricular Zone-Derived Neural Stem Cells

01:46 min

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29 Views

Esteban Jiménez-Villalba*¹, Laura Lázaro-Carot*¹, Carmen M. Mateos-Martínez¹, Jennifer Díaz-Moncho¹, Daniel Samper-Llavador¹, Marta Igual-López¹, Jordi Planells¹, Sacri R. Ferrón¹

¹Instituto de Biotecnología y Biomedicina (BIOTECMED), Departamento de Biología Celular, Biología Funcional y Antropología Física,Universitat de València

Summary

Here, we describe a nucleofection system designed to enhance gene delivery efficiency in expanded neural stem cells (NSCs) isolated from the adult murine subventricular zone. The findings demonstrate that this method significantly improves gene perturbation in NSCs, surpassing the effectiveness of traditional transfection protocols and enhancing cell survival rate.