Construction of Out&#45;of&#45;Equilibrium Metabolic Networks in Nano&#45; and Micrometer&#45;Sized Vesicles

Jelmer Coenradij; Eleonora Bailoni; Bert Poolman

doi:10.3791/66627

Journal

Biochimie

Lipid Vesicle Encapsulation Protocol for Complex Protein Networks

April 12, 2024

Author Spotlight: Tackling Challenges in Synthetic Cell Engineering

02:16 min

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374 Views

Reconstitution of Purified Membrane Proteins into Preformed Liposomes

02:56 min

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90 Views

Encapsulation of a Metabolic Network for ATP Recycling and Glycerol 3-P Synthesis in Submicron-Size Vesicles

01:58 min

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49 Views

Upscaling a Metabolic Network for Micrometer-Sized Vesicles

03:51 min

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48 Views

Jelmer Coenradij¹, Eleonora Bailoni¹, Bert Poolman¹

¹Department of Biochemistry,University of Groningen

Summary

We present a protocol for reconstituting membrane proteins and encapsulating enzymes and other water-soluble components in lipid vesicles of sub-micrometer and micrometer size.