Real-Time Imaging of Acrosomal Calcium Dynamics and Exocytosis in Live Mouse Sperm

Roy Cohen; Danielle M. Sosnicki; Melissa A. White; Jacquelyn L. Nelson; Chinatsu Mukai; Alexander J. Travis

doi:10.3791/65962

Journal

Biologie du développement

Combining Imaging with a Stimulant Delivery System to Study Live Mouse Sperm

October 13, 2023

Author Spotlight: A Live Cell Imaging Technique to Study Calcium Signaling and Acrosome Exocytosis in Mouse Sperm

00:46 min

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559 Views

Collection and Washing of Sperm from the AcroSensE Mouse Model for Real-Time Imaging

01:27 min

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66 Views

Setting up the Single Cell Stimulant Delivery System for Real-Time Monitoring of Live Mouse Sperm

01:19 min

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36 Views

Combining Microscopy with a Stimulant Delivery System to Monitor Live Sperm from an AcroSensE Model Mouse

01:45 min

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41 Views

Roy Cohen^1,2, Danielle M. Sosnicki¹, Melissa A. White¹, Jacquelyn L. Nelson¹, Chinatsu Mukai¹, Alexander J. Travis^1,2

¹Baker Institute for Animal Health, College of Veterinary Medicine,Cornell University, ²Department of Public and Ecosystem Health, College of Veterinary Medicine,Cornell University

Summary

The AcroSensE mouse model and live cell imaging methods described here provide a new approach to studying calcium dynamics in the subcellular compartment of the sperm acrosome and how they regulate intermediate steps leading to membrane fusion and acrosome exocytosis.