Visualizing Single-Stranded DNA Foci in the G1 Phase of the Cell Cycle

Qingyue Zhang; Marc A. Kerzhnerman; Nelson Garc&#237;a-V&#225;zquez; Gergely Rona

doi:10.3791/65926

Journal

Biologie

Detection of G1 Phase Single-Stranded DNA Foci via RPA2 Immunofluorescent Staining

December 22, 2023

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Qingyue Zhang^1,2, Marc A. Kerzhnerman^1,2, Nelson García-Vázquez^1,2,3, Gergely Rona^1,2,4,5

¹Department of Biochemistry and Molecular Pharmacology,NYU Grossman School of Medicine, ²The Laura and Isaac Perlmutter Cancer Center,NYU Langone Health, ³Department of Cell Biology,NYU Grossman School of Medicine, ⁴Howard Hughes Medical Institute,NYU Grossman School of Medicine, ⁵Institute of Enzymology, Centre of Excellence of the Hungarian Academy of Sciences,HUN-REN Research Centre for Natural Sciences

Summary

The following protocol presents the detection of single-stranded DNA foci in the G1 phase of the cell cycle utilizing cell cycle synchronization followed by RPA2 immunofluorescent staining.

Detection of G1 Phase Single-Stranded DNA Foci via RPA2 Immunofluorescent Staining

Author Spotlight: Visualizing Single-Stranded DNA During DNA Repair for Therapeutic Insights

Preparation of G1 Synchronized Cells to Measure ssDNA Foci Formation upon Genotoxic Stress

Summary

Article