Journal
Immunologie et infection
PBMC Collection and Cryostorage for Mitochondrial Bioenergetics Analysis

PBMC Collection and Cryostorage for Mitochondrial Bioenergetics Analysis

October 20, 2023

Author Spotlight: Preservation of Bioenergetic Parameters in Peripheral Blood Mononuclear Cells After Cryopreservation

01:27 min
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569 Views

Isolation and Cryopreservation of Human Peripheral Blood Mononuclear Cells for Bioenergetic Studies

02:46 min
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167 Views
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1Biomedical Research Center, Institute of Nutritional Sciences,Justus-Liebig-University of Giessen

Summary

Isolated peripheral blood mononuclear cells can be used for the analysis of immune functions and disorders, metabolic diseases, or mitochondrial functions. In this work, we describe a standardized method for the preparation of PBMCs from whole blood and the subsequent cryopreservation. Cryopreservation makes this time and place independent.

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